NIMA-Related Kinase 1 (NEK1) (C-Term), (AA 1165-1196) antibody

Details for Product No. ABIN392648
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Synonyms NY-REN-55, SRPS2, SRPS2A, D8Ertd790e, kat, ATNEK1, F20D21.32, F20D21_32, NIMA-RELATED KINASE1, NIMA-related serine/threonine kinase 1
C-Term, AA 1165-1196
(2), (1), (1), (1)
Human, Hamster
(15), (11), (11), (11), (11), (2)
Clonality (Clone)
Polyclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunohistochemistry (IHC)
(9), (7), (5), (4)
Pubmed 2 references available
Catalog no. ABIN392648
Quantity 400 µL
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Immunogen This NEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1165-1196 AA from the C-terminal region of human NEK1.
Clone RB2957
Isotype Ig
Specificity This NEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1173~1202 amino acids from the C-terminal region of human NEK1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name NEK1
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Synonyms: Serine/threonine-protein kinase Nek1, NimA-related protein kinase 1, NY-REN-55 antigen
Molecular Weight 142828 DA
Gene ID 4750
UniProt Q96PY6
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-NIMA-Related Kinase 1 (NEK1) (C-Term), (AA 1165-1196) antibody The anti-NEK1 Pab (ABIN392648) is used in Western blot to detect NEK1 in CHO cell lysate.
anti-NIMA-Related Kinase 1 (NEK1) (C-Term), (AA 1165-1196) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Letwin, Mizzen, Motro et al.: "A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells." in: The EMBO journal, Vol. 11, Issue 10, pp. 3521-31, 1992 (PubMed).

Surpili, Delben, Kobarg: "Identification of proteins that interact with the central coiled-coil region of the human protein kinase NEK1." in: Biochemistry, Vol. 42, Issue 51, pp. 15369-76, 2003 (PubMed).

Hosts (16)
Reactivities (15), (11), (11), (11), (11), (2)
Applications (9), (7), (5), (4)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (1), (1), (1)
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