NEK1 antibody (C-Term)

Catalog No. ABIN360536

The Rabbit Polyclonal anti-NEK1 antibody is suitable to detect NEK1 in samples from Human and Hamster. It has been validated for WB, IHC (p) and EIA.

Quick Overview for NEK1 antibody (C-Term) (ABIN360536)

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Reactivity: Human, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-NEK1 Antibody

Binding Specificity: AA 1173-1202, C-Term

Specificity: This antibody is specific to NEK1.

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 1173~1202 amino acids from the C-terminal region of Human NEK1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for NEK1

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Alternative Name: NEK1

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: NimA-related protein kinase 1, NimA-related protein kinase 10, Renal carcinoma antigen NY-REN-55, Serine/threonine-protein kinase Nek1

Gene ID: 4750

NCBI Accession: NP_001186326

UniProt: Q96PY6