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PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (AA 58-81) antibody

Details for Product No. ABIN400795, Supplier: Log in to see
Antigen
  • HNPCC4
  • PMS2CL
  • PMSL2
  • AW555130
  • Pmsl2
Alternatives
anti-Human PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) antibody for Western Blotting
Epitope
AA 58-81
11
7
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Chimpanzee, Hamster, Human, Mouse (Murine), Rat (Rattus)
80
15
11
1
1
1
1
1
1
Host
Mouse
52
28
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
52
39
21
17
12
8
2
1
1
1
1
Supplier
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Immunogen Recombinant human PMS2 corresponding to the first 133 amino acid residues of theprotein
Clone 349-29-5-2
Isotype IgG1
Specificity This antibody is directed against PMS2-134 and reacts with full length version of PMS2. The epitope was putatively mapped to amino acids 58-81 of human PMS2. Expect a band approximately 96 kDa in size corresponding to human PMS2 by western blotting in most cell lines and tissues as PMS2 is ubiquitously expressed.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Chimpanzee (100 %)Species reactivity (tested):Human, Hamster
Characteristics Synonyms: PMSL2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2
Purification Protein chromatography
Alternative Name PMS2 (PMS2 Antibody Abstract)
Background PMS2 is a highly conserved nuclear protein involved in mismatch repair during DNA replication and has been identified to be composed as a heterodimer of PMS2 and MLH1. PMS is part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Turcot syndrome (an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas) and supratentorial primitive neuroectodermal tumors with cafe-au-lait spots (SNTCL). The human PMS2 gene encodes an 862 aa, 96 kDa polypeptide.Synonyms: DNA mismatch repair protein PMS2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2, PMSL2
Gene ID 5395
UniProt P54278
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Pathways DNA Damage Repair
Application Notes ELISA: 1: 5,000 - 1: 20,000. Western blot: 1: 500 - 1: 2,000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.38 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 a buffer and 0.09 % (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (AA 58-81) antibody (ABIN400795) anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (AA 58-81) antibody
 image for anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (AA 58-81) antibody (ABIN400795) anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (AA 58-81) antibody (Image 2)
Background publications Nakagawa, Lockman, Frankel et al.: "Mismatch repair gene PMS2: disease-causing germline mutations are frequent in patients whose tumors stain negative for PMS2 protein, but paralogous genes obscure mutation detection and interpretation." in: Cancer research, Vol. 64, Issue 14, pp. 4721-7, 2004 (PubMed).

Luo, Lin, Lin: "ATM-mediated stabilization of hMutL DNA mismatch repair proteins augments p53 activation during DNA damage." in: Molecular and cellular biology, Vol. 24, Issue 14, pp. 6430-44, 2004 (PubMed).

de Jong, van Puijenbroek, Hendriks et al.: "Microsatellite instability, immunohistochemistry, and additional PMS2 staining in suspected hereditary nonpolyposis colorectal cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 3, pp. 972-80, 2004 (PubMed).

Yuan, Gottlieb, Beitel et al.: "Polymorphisms and HNPCC: PMS2-MLH1 protein interactions diminished by single nucleotide polymorphisms." in: Human mutation, Vol. 19, Issue 2, pp. 108-13, 2002 (PubMed).

Wang, Cortez, Yazdi et al.: "BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures." in: Genes & development, Vol. 14, Issue 8, pp. 927-39, 2000 (PubMed).

Nicolaides, Papadopoulos, Liu et al.: "Mutations of two PMS homologues in hereditary nonpolyposis colon cancer." in: Nature, Vol. 371, Issue 6492, pp. 75-80, 1994 (PubMed).

Zankl, Schwanitz, Schmid et al.: "Distal 2q duplication: report of two familial cases and an attempt to define a syndrome." in: American journal of medical genetics, Vol. 4, Issue 1, pp. 5-16, 1979 (PubMed).