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PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (AA 58-81) antibody

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Synonyms HNPCC4, PMS2CL, PMSL2, AW555130, Pmsl2
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AA 58-81
(11), (7), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Chimpanzee, Hamster, Human, Mouse (Murine), Rat (Rattus)
(86), (15), (11), (1), (1), (1), (1), (1), (1)
(57), (29)
Clonality (Clone)
Monoclonal ()
(2), (2), (1)
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(57), (39), (21), (18), (13), (11), (2), (2), (1), (1), (1)
Pubmed 7 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Recombinant human PMS2 corresponding to the first 133 amino acid residues of theprotein
Clone 349-29-5-2
Isotype IgG1
Specificity This antibody is directed against PMS2-134 and reacts with full length version of PMS2. The epitope was putatively mapped to amino acids 58-81 of human PMS2. Expect a band approximately 96 kDa in size corresponding to human PMS2 by western blotting in most cell lines and tissues as PMS2 is ubiquitously expressed.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Chimpanzee (100 % )Species reactivity (tested):Human, Hamster
Characteristics Synonyms: PMSL2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2
Purification Protein chromatography
Alternative Name PMS2 (PMS2 Antibody Abstract)
Background PMS2 is a highly conserved nuclear protein involved in mismatch repair during DNA replication and has been identified to be composed as a heterodimer of PMS2 and MLH1. PMS is part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Turcot syndrome (an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas) and supratentorial primitive neuroectodermal tumors with cafe-au-lait spots (SNTCL). The human PMS2 gene encodes an 862 aa, 96 kDa polypeptide.Synonyms: DNA mismatch repair protein PMS2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2, PMSL2
Gene ID 5395
UniProt P54278
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Pathways DNA Damage Repair
Application Notes ELISA: 1: 5,000 - 1: 20,000. Western blot: 1: 500 - 1: 2,000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.38 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 a buffer and 0.09 % (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (AA 58-81) antibody (ABIN400795) anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (AA 58-81) antibody
 image for anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (AA 58-81) antibody (ABIN400795) anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) (AA 58-81) antibody (Image 2)
Background publications Nakagawa, Lockman, Frankel et al.: "Mismatch repair gene PMS2: disease-causing germline mutations are frequent in patients whose tumors stain negative for PMS2 protein, but paralogous genes obscure mutation detection and interpretation." in: Cancer research, Vol. 64, Issue 14, pp. 4721-7, 2004 (PubMed).

Luo, Lin, Lin: "ATM-mediated stabilization of hMutL DNA mismatch repair proteins augments p53 activation during DNA damage." in: Molecular and cellular biology, Vol. 24, Issue 14, pp. 6430-44, 2004 (PubMed).

de Jong, van Puijenbroek, Hendriks et al.: "Microsatellite instability, immunohistochemistry, and additional PMS2 staining in suspected hereditary nonpolyposis colorectal cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 3, pp. 972-80, 2004 (PubMed).

Yuan, Gottlieb, Beitel et al.: "Polymorphisms and HNPCC: PMS2-MLH1 protein interactions diminished by single nucleotide polymorphisms." in: Human mutation, Vol. 19, Issue 2, pp. 108-13, 2002 (PubMed).

Wang, Cortez, Yazdi et al.: "BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures." in: Genes & development, Vol. 14, Issue 8, pp. 927-39, 2000 (PubMed).

Nicolaides, Papadopoulos, Liu et al.: "Mutations of two PMS homologues in hereditary nonpolyposis colon cancer." in: Nature, Vol. 371, Issue 6492, pp. 75-80, 1994 (PubMed).

Zankl, Schwanitz, Schmid et al.: "Distal 2q duplication: report of two familial cases and an attempt to define a syndrome." in: American journal of medical genetics, Vol. 4, Issue 1, pp. 5-16, 1979 (PubMed).

Catalog No. ABIN400795
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