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Biotin antibody (FITC)

FACS, IF, IHC (fro) Host: Rabbit Polyclonal FITC

Catalog No. ABIN401080

Datasheet as PDF

Target See all Biotin products

Biotin

Reactivity

Please inquire
Host
63

38

30
Rabbit
Compatible Secondaries
Clonality
83

40

1
Polyclonal
Conjugate
35

14

8

8

8

6

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Biotin antibody is conjugated to FITC
Application
78

56

51

47

39

33

16

13

11

9

6

5

4

4

3

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Characteristics

Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 2.7 Moles Fluorescein (FITC) per mole of IgG

Immunogen

Biotin conjugated Keyhole Limpet Hemocyanin (KLH)

Isotype

IgG
anti-Biotin antibody
WB, ELISA, FACS, IHC, IF, FM Host: Mouse Monoclonal 4C7-D5 unconjugated

anti-Biotin antibody
WB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated

anti-Biotin antibody
IHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody

anti-Biotin antibody
WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Biotin antibody (DyLight 488)
WB, FACS, IF, FM, FLISA, DB, MA Host: Goat Polyclonal DyLight 488

anti-Biotin antibody
ELISA, FACS Host: Mouse Monoclonal SB58c unconjugated

anti-Biotin antibody
WB, ELISA, FACS, IHC, IP, Lbl Host: Goat unconjugated

anti-Biotin antibody
ELISA, FACS, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
Reactivity: Human WB, ELISA, IHC Host: Mouse Monoclonal B-E24 unconjugated

anti-Biotin antibody
Reactivity: Human WB Host: Rabbit Monoclonal unconjugated

Application Notes

Immunofluorescence. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Reconstitution

Restore with 2.0 mL of deionized water (or equivalent).

Concentration

10.0 mg/mL (by UV absorbance at 280 nm)

Buffer

0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.4, 0.01 % (w/v) Thimerisol, 10 mg/mL BSA (IgG and Protease free)

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target

Biotin

Abstract

Biotin Products

Target Type

Chemical

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.