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Details for Product No. ABIN401240

HA-Tag antibody

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Antigen
Reactivity
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN401240
Quantity 0.1 mg
Price
324.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from Hemagglutinin Influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Isotype IgG
Specificity This affinity purified antibody is directed against the HA motif and is useful in determining its presence in various assays. This anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, it has reacted with all HA-tagged proteins tested. In Western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Purification Affinity Chromatography
Alternative Name HA Epitope Tag (YPYDVPDYA)
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti- epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Application Notes Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. Theantibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targetedproteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and Western blotting against both the immunizingpeptide and HA containing recombinant proteins. This antibody is likely functional forImmunoprecipitation, Immunocytochemistry, and other Immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels inassays and low cross-reactivity with other cellular proteins. Recommended Dilutions: ELISA: 1/20,000-1/150,000.
Restrictions For Research Use only
Format Liquid
Concentration 0.95 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Background publications Field, Nikawa, Broek et al.: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

Alternatives for antigen "HA-Tag", type "Antibodies"
Hosts (124), (115), (45), (25), (3), (1)
Reactivities (257), (21), (19), (17), (6), (6), (2), (2), (1), (1)
Applications (243), (154), (105), (66), (50), (42), (30), (19), (13), (12), (9), (6), (6), (4), (4), (3), (2), (2), (2), (1)
Conjugates (30), (20), (18), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Epitopes (10), (2), (1)
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