GLI2 antibody (Isoform alpha)

Catalog No. ABIN401285

Product Details anti-GLI2 Antibody

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Binding Specificity: AA 40-60, AA 46-60, Isoform alpha

Reactivity: Human, Mouse, Rat, Chimpanzee, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against Gli-2 protein. Expect reactivity with all isoforms of this protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee (100 %), Mouse and Rat (93 %), Bovine (86 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 46-60 of Human Gli-2 (isoform alpha).

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2,000-1/12,000. Western blot: 1/500-1/2,000. Detection of Gli-2 may be enhanced if nuclear extracts areused instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced,making it difficult to detect in whole tissue homogenates. Immunohistochemistry on Paraffin Sections: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Alternative Name: GLI2 (GLI2 Products)

Synonyms: HPE9 antibody, THP1 antibody, THP2 antibody, GLI2/GLI4 antibody, GLI3 antibody, AW546128 antibody, GLI family zinc finger 2 antibody, GLI-Kruppel family member GLI2 antibody, GLI2 antibody, Gli2 antibody

Background: Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is a nuclear protein thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes, Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. Multiple splice variants have been reported for this protein.Synonyms: GLI-2, THP, Tax helper protein, Zinc finger protein GLI2

Gene ID: 2736

NCBI Accession: NP_005261

UniProt: P10070

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis