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DARPP32 antibody (pThr75)

PPP1R1B Reactivity: Human, Mouse, Rat IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN401601
  • Target See all DARPP32 (PPP1R1B) Antibodies
    DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
    Binding Specificity
    • 33
    • 17
    • 15
    • 9
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr75
    Reactivity
    • 95
    • 84
    • 75
    • 13
    • 12
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 109
    • 11
    • 1
    • 1
    • 1
    • 1
    Rabbit
    Clonality
    • 103
    • 21
    Polyclonal
    Conjugate
    • 73
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This DARPP32 antibody is un-conjugated
    Application
    • 94
    • 38
    • 35
    • 28
    • 27
    • 18
    • 17
    • 8
    • 5
    • 4
    • 3
    • 1
    Immunofluorescence (IF)
    Specificity
    DARPP-32 (Phospho-Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 75.
    Purification
    Affinity chromatography
    Immunogen
    Synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).
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    Discover our top product PPP1R1B Primary Antibody
  • Application Notes
    Immunofluorescence: 1/100 - 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1,0 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
  • Target
    DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
    Alternative Name
    DARPP32 (PPP1R1B Products)
    Synonyms
    darpp32 antibody, darpp-32 antibody, Darpp-32 antibody, Darpp32 antibody, DARPP-32 antibody, DARPP32 antibody, AU040756 antibody, protein phosphatase 1 regulatory inhibitor subunit 1B antibody, protein phosphatase 1, regulatory (inhibitor) subunit 1B antibody, protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog antibody, ppp1r1b antibody, PPP1R1B antibody, Ppp1r1b antibody, ppp1r1b.S antibody
    Background
    DARPP32 is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32 and phosphorylated DARPP32 is a potent protein phosphatase 1. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho DARPP32, thereby reducing the phosphatase 1 inhibitory activity of DARPP32.Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B
    Gene ID
    84152
    NCBI Accession
    NP_115568
    UniProt
    Q9UD71
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