rho GTPase Activating Protein 25 (ARHGAP25) (Middle Region) antibody

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Antigen
Synonyms arhgap24, DKFZp468H165, KAIA0053, A130039I20Rik, RGD1562105
Epitope
Middle Region
(15), (14), (2), (2), (2), (1), (1), (1)
Reactivity
Cow (Bovine), Human
(82), (12), (10), (1)
Host
Rabbit
(43), (39)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(78), (37), (32), (32), (23), (21), (18), (3)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the middle region of human ARHGAP25
Sequence SPGEEASALS SQACDSKGDT LASPNSETGP GKKNSGEEEI DSLQRMVQEL
Predicted Reactivity Human : 100 %, Bovine : 90 %, Dog : 90 %, Horse : 90 %, Rabbit : 90 %, Pig : 80 %
Characteristics This is a rabbit polyclonal antibody against ARHGAP25. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name ARHGAP25 (ARHGAP25 Antibody Abstract)
Background ARHGAPs, such as ARHGAP25, are negative regulators of Rho GTPases, which are implicated in actin remodeling, cell polarity, and cell migration.ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA, MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).
Molecular Weight 70 kDa
Gene ID 9938
NCBI Accession NP_055697, NM_014882
UniProt P42331
Research Area Signaling
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 638 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-rho GTPase Activating Protein 25 (ARHGAP25) (Middle Region) antibody (ABIN406687) WB Suggested Anti-ARHGAP25 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Background publications Katoh, Katoh: "Identification and characterization of ARHGAP24 and ARHGAP25 genes in silico." in: International journal of molecular medicine, Vol. 14, Issue 2, pp. 333-8, 2004 (PubMed).

Catalog No. ABIN406687
$289.00
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