Epithelial Cell Adhesion Molecule (EPCAM) antibody

Details for Product No. ABIN452356
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DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, EGP, Ep-CAM, CD326, Egp314, EpCAM1, GA733-2, Ly74, Tacsd1, Tacstd1, gp40, sb:cb6, tacstd, wu:fj17g02, zgc:11030 ... show more
DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, EGP, Ep-CAM, CD326, Egp314, EpCAM1, GA733-2, Ly74, Tacsd1, Tacstd1, gp40, sb:cb6, tacstd, wu:fj17g02, zgc:110304, zgc:77119, tacstd1, MGC80540, EPCAM, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2 show less
(223), (52), (39), (17), (17), (17), (2)
(173), (58), (12)
Clonality (Clone)
Monoclonal ()
(18), (8), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(126), (114), (76), (50), (45), (39), (34), (34), (29), (20), (5), (5), (3), (2), (2), (2)
Pubmed 6 references available
Catalog no. ABIN452356
Quantity 5 mL
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Immunogen HT-29 colon carcinoma cell line.
Clone HEA125
Isotype IgG1
Specificity Clone HEA125 recognizes Ep-CAM (polypeptide reacting: Mr 40,000 epithelium-specific cell surface glycoprotein). HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far, particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116). Polypeptide Reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep- CAM). Species Reactivity: Tested: Human.
Purification Affinity Chromatography on Protein A
Alternative Name CD326 / EPCAM / TACSTD1
Background This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
Alternate names: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
Gene ID 4072
NCBI Accession NP_002345
UniProt P16422
Research Area Cancer
Application Notes Immunoblotting. Cell sorting. Cytological Material. Immunofluorescence microscopy. Immunohistochemistry on Frozen and Paraffin-Embedded Tissue. Pretreatment: With paraffin embedded sections, protease pre-treatment is required prior toantibody application. Working Dilutions: Ready-to-use for immunohistochemical application.
Restrictions For Research Use only
Format Liquid
Buffer PBS, pH 7.4 0.09% Sodium Azide 0.5% BSA
Preservative Sodium azide
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month. DO NOT FREEZE!
Expiry Date 12 months
Background publications Harris: "Mutant p53--the commonest genetic abnormality in human cancer?" in: The Journal of pathology, Vol. 162, Issue 1, pp. 5-6, 1990 (PubMed).

Hastka, Pfiester: "[Cell marker for the differential diagnosis of malignant mesotheliomas]." in: Der Pathologe, Vol. 9, Issue 4, pp. 245-7, 1989 (PubMed).

Hamburger, White, Dunn: "Secretion of transforming growth factors by primary human tumour cells." in: British journal of cancer, Vol. 51, Issue 1, pp. 9-14, 1985 (PubMed).

Racila, Euhus, Weiss et al.: "Detection and characterization of carcinoma cells in the blood." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4589-94, 1998 (PubMed).

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).

Bechtel, Petty, Saccomanno: "Five year survival and later outcome of patients with X-ray occult lung cancer detected by sputum cytology." in: Lung cancer (Amsterdam, Netherlands), Vol. 30, Issue 1, pp. 1-7, 2005 (PubMed).

Hosts (173), (58), (12)
Reactivities (223), (52), (39), (17), (17), (17), (2)
Applications (126), (114), (76), (50), (45), (39), (34), (34), (29), (20), (5), (5), (3), (2), (2), (2)
Conjugates (18), (8), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Epitopes (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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