Chemokine (C-C Motif) Ligand 2 (CCL2) antibody (HRP)

Details for Product No. ABIN453918
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Antigen
Synonyms MCP-1, Scya2, Sigje, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, AI323594, JE, MCP-1A, MCP1A
Reactivity
Human
(207), (95), (85), (17), (17), (15), (6), (5), (4), (2), (2), (1), (1)
Host
Mouse
(187), (100), (21), (6), (4), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
(35), (18), (12), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
ELISA
(238), (205), (61), (41), (33), (33), (28), (28), (21), (20), (16), (15), (5), (5), (2), (1), (1)
Pubmed 9 references available
Quantity 0.2 mL
Shipping to United States (Change)
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Catalog No. ABIN453918
533.50 $
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Immunogen Purified recombinant Human MCP1.
Clone S101
Isotype IgG1
Specificity This monoclonal antibody reacts with natural and recombinant Human MCP-1. Does not react with Human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1 beta (IL1 beta), serum amyloid A (SAA) and epidermal growth factor (EGF) Species Reactivity: Tested: Human.
Purification Affinity Chromatography on Protein G
Alternative Name MCP1 / CCL2
Background Monocyte chemotactic protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or beta) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro- inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G-protein coupled receptor CCR2 (9).
Alternate names: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2
Gene ID 6347
NCBI Accession NP_002973
UniProt P13500
Research Area Cytokines, Chemokines
Application Notes ELISA: This MCP1 antibody HRP-conjugated can be used as a Tracer/Detection antibody inSandwich ELISA applications for Human MCP-1 detection in combination with a Captureantibody (Cat#DM2032)Suggested Capture Coating Dose: ~0.3 µg/mlSubstrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL ofMCP-1 in serum/plasma or 4 pg/ml of MCP-1 in medium can be detected with an assayrange of 0-1600 pg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 0.01M PBS, pH 7.0 ± 0.1 in 50% Glycerol and 0.01% Thimerosal as a bacteriostat
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Background publications Koch, Kunkel, Harlow et al.: "Enhanced production of monocyte chemoattractant protein-1 in rheumatoid arthritis." in: The Journal of clinical investigation, Vol. 90, Issue 3, pp. 772-9, 1992 (PubMed).

Ylä-Herttuala, Lipton, Rosenfeld et al.: "Expression of monocyte chemoattractant protein 1 in macrophage-rich areas of human and rabbit atherosclerotic lesions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 12, pp. 5252-6, 1991 (PubMed).

Robinson, Yoshimura, Leonard et al.: "Complete amino acid sequence of a human monocyte chemoattractant, a putative mediator of cellular immune reactions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 6, pp. 1850-4, 1989 (PubMed).

Furutani, Nomura, Notake et al.: "Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF)." in: Biochemical and biophysical research communications, Vol. 159, Issue 1, pp. 249-55, 1989 (PubMed).

Matsushima, Larsen, DuBois et al.: "Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1485-90, 1989 (PubMed).

Rollins, Morrison, Stiles: "Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 11, pp. 3738-42, 1988 (PubMed).

Charo, Myers, Herman et al.: "Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 7, pp. 2752-6, 1994 (PubMed).

Gillitzer, Wolff, Tong et al.: "MCP-1 mRNA expression in basal keratinocytes of psoriatic lesions." in: The Journal of investigative dermatology, Vol. 101, Issue 2, pp. 127-31, 1993 (PubMed).

Salcedo, Ponce, Young et al.: "Human endothelial cells express CCR2 and respond to MCP-1: direct role of MCP-1 in angiogenesis and tumor progression." in: Blood, Vol. 96, Issue 1, pp. 34-40, 2000 (PubMed).

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