BAFF antibody (AA 83-285)

Catalog No. ABIN461787

Product Details anti-BAFF Antibody

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Binding Specificity: AA 83-285

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This BAFF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes membrane-bound human BAFF. Detects endogenous protein by FC.

Purification: Gel filtration

Immunogen: Recombinant human soluble BAFF (aa 83-285).

Type of Immunogen: Recombinant protein

Clone: Buffy-1

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, ICC, IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for BAFF

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: BAFF (TNFSF13B Products)

Background: Name/Gene ID: TNFSF13B
Family: TNF

Synonyms: TNFSF13B, B-cell-activating factor, BAFF, ApoL related ligand TALL-1, B-lymphocyte stimulator, CD257, BLYS, Delta4 BAFF, Delta BAFF, TALL-1, TALL1, ZTNF4, THANK, B lymphocyte stimulator, CD257 antigen, TNFSF20

Gene ID: 10673

UniProt: Q9Y275

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response