Adenosine A2a Receptor antibody

Catalog No. ABIN487435

Product Details anti-Adenosine A2a Receptor Antibody

Target: Adenosine A2a Receptor (ADORA2A)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Adenosine A2a Receptor antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody recognizes Human Adenosine Receptor A2A at ~43 kDa. Does not recognize Adenosine Receptor A2A in knockout mice.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Guinea Pig and Rabbit.
Species reactivity (tested):Human.

Characteristics: Synonyms: ADORA2A, ADORA2

Purification: Protein G Chromatography

Immunogen: 6His-tagged full-length Human recombinant A2A receptor. Genename: ADORA2ARemarks: Epitope mapped to SQPLPGER in the third intracellular loop.

Clone: N-n

Isotype: IgG2a

Application Details

Application Notes: Western blot: 1/1000-1/5000. Flow Cytometry. Immunohistochemistry (Paraffin Sections): 5 μg/mL. Immunohistochemistry (4 % Paraformaldehyde): 1 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.01 M Tris-Glycine, pH 7.4, 0.11 M sodium chloride, 0.05 % sodium azide.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Alternative Name: Adenosine Receptor A2a (ADORA2A Products)

Background: The A2a Adenosine Receptor is a major target of caffeine. This receptor inhibits cell aggregation, induces vasodilation, and downregulates inflammation. This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.Synonyms: ADORA2, ADORA2A

Gene ID: 135

UniProt: P29274

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints