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EGFR antibody (Ligand Binding Domain)

EGFR Reactivity: Human FACS, IHC (p), EIA Host: Mouse Monoclonal EGF-R1 unconjugated

Catalog No. ABIN492656

Datasheet as PDF

Target See all EGFR Antibodies

EGFR (Epidermal Growth Factor Receptor (EGFR))
Binding Specificity
39

38

31

31

30

27

27

26

24

22

19

18

16

16

16

15

15

15

11

11

10

9

9

9

8

8

7

6

5

5

5

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3
Ligand Binding Domain
Reactivity
748

296

283

25

25

24

19

14

8

3

2

2

1

1

1

1

1

1
Human
Host
580

167

14

13

5

2

1

1
Mouse
Compatible Secondaries
Clonality
542

236

2
Monoclonal
Conjugate
410

58

29

22

19

17

16

16

16

16

16

11

10

10

10

10

10

10

10

10

10

8

8

5

4

4

4

4

4

1

1

1

1

1

1
This EGFR antibody is un-conjugated
Application
535

248

149

145

126

102

98

93

82

46

37

34

34

18

11

8

6

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity

Recognizes Human EGF-Receptor (extracellular ligand binding site). There was no cross reactivity obtained with v-erb-B.

Characteristics

Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification

Protein G Chromatography

Immunogen

Human EGF-Receptor

Clone

EGF-R1

Isotype

IgG1

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anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibody
EGFR Reactivity: Human, Rat WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA (1, 2)Flow Cytometry. Immunohistochemistry on Paraffin Sections (1.7 ng/mL PBS, 1 % BSA).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Reconstitution

Restore in aqua bidest to 1 mg/mL

Buffer

PBS pH 7.4, 0.09 % Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store lyophilized at 2-8 °C and reconstituted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date

12 months
Target

EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name

EGFR / ERBB1 (EGFR Products)

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

Gene ID

1956

UniProt

P00533

Pathways

NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins