Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN497967

ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) (N-Term) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
ASP, APG5, APG5L, hAPG5, APG5-LIKE, Apg5l, Atg5l, Paddy, C88337, AW319544, 2010107M05Rik, 3110067M24Rik, apg5l, zgc:100934, ATATG5, ATG5, AUTOPHAGY 5, MKP11.20, MKP11_20, CG1643, DmAtg5, Dmel\\CG1643, ... show more
Epitope
»Alternatives N-Term
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Chicken
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN497967
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Chicken polyclonal ATG5 antibody was raised against a 16 amino acid peptide from near the amino terminus of Human ATG5.
Specificity Recognizes APG5L/ATG5.
Purification Affinity Chromatography purified via peptide column.
Alternative Name APG5L / ATG5
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Three isoforms of ATG5 are known to exist, this ATG5 antibody will only detect the longest isoform.
Alternate names: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5
Gene ID 9474
NCBI Accession NP_004840
UniProt Q9H1Y0
Application Notes ELISA. Western Blot: ATG5 antibody can be used for the detection of ATG5 at 1–2 μg/ml. Positive Control. Mouse Spleen Tissue Lysate. Immunohistochemistry. Positive Control. Human Spleen Tissue Lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% Sodium Azide as preservative.
Preservative Sodium azide
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C. Antibodies should not be exposed to prolonged high temperatures.
Expiry Date 12 months
Background publications Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Kamada, Funakoshi, Shintani et al.: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Jounai, Takeshita, Kobiyama et al.: "The Atg5 Atg12 conjugate associates with innate antiviral immune responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 35, pp. 14050-5, 2007 (PubMed).

Hanada, Noda, Satomi et al.: "The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy." in: The Journal of biological chemistry, Vol. 282, Issue 52, pp. 37298-302, 2007 (PubMed).

Alternatives for antigen "ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5)", type "Antibodies"
Hosts (97), (24), (13), (4), (3)
Reactivities (127), (71), (59), (37), (33), (18), (17), (16), (13), (13), (2), (1), (1), (1), (1)
Applications (124), (55), (54), (24), (20), (10), (10), (6), (3), (2), (1), (1)
Conjugates (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (31), (11), (10), (10), (4), (4), (2), (1), (1), (1), (1), (1), (1)
Beacon