MARCH8 antibody (C-Term)
-
- Target See all MARCH8 Antibodies
- MARCH8 (Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8))
-
Binding Specificity
- C-Term
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This MARCH8 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- Specificity
- This antibody detects MARCH8.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human, mouse, rat
- Purification
- Peptide affinity chromatography
- Immunogen
- MARCH8 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human MARCH8.
- Isotype
- IgG
- Top Product
- Discover our top product MARCH8 Primary Antibody
-
-
- Application Notes
-
ELISA. Western blot: 0.5 - 1 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Buffer
- PBS containing 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
-
- Target
- MARCH8 (Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8))
- Alternative Name
- MARCH8 (MARCH8 Products)
- Synonyms
- MARCH-VIII antibody, MIR antibody, RNF178 antibody, c-MIR antibody, zgc:171495 antibody, 1300017E09Rik antibody, Mir antibody, Mar-08 antibody, MARCH8 antibody, membrane associated ring-CH-type finger 8 antibody, membrane-associated ring finger (C3HC4) 8 antibody, membrane associated ring-CH-type finger 8 L homeolog antibody, MARCH8 antibody, march8 antibody, March8 antibody, march8.L antibody
- Background
- MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.Synonyms: Cellular modulator of immune recognition, E3 ubiquitin-protein ligase MARCH8, MARCH-VIII, Membrane-associated RING finger protein 8, RING finger protein 178, RNF178, c-MIR
- Gene ID
- 220972
- NCBI Accession
- NP_659458
- UniProt
- Q5T0T0
-