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Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8) (C-Term) antibody

Details for Product No. ABIN500234
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Antigen
Synonyms 1300017E09Rik, Mir, zgc:171495, c-MIR, MARCH-VIII, MIR, RNF178, Mar-08
Epitope
C-Term
(8), (2), (2), (2), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(37), (27), (27), (18), (18), (17), (1), (1)
Host
Rabbit
(38), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(26), (15), (14), (13), (10), (7), (1)
Pubmed 4 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN500234
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Immunogen MARCH8 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human MARCH8.
Isotype IgG
Specificity This antibody detects MARCH8. Species Reactivity: Tested: Human, mouse, rat
Purification Peptide affinity chromatography
Alternative Name MARCH8
Background MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.
Alternate names: Cellular modulator of immune recognition, E3 ubiquitin-protein ligase MARCH8, MARCH- VIII, Membrane-associated RING finger protein 8, RING finger protein 178, RNF178, c-MIR
Gene ID 220972
NCBI Accession NP_659458
UniProt Q5T0T0
Research Area Proteolysis / Ubiquitin, Adaptive Immunity
Application Notes ELISA. Western blot: 0.5 – 1 μg/ml. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Coscoy, Sanchez, Ganem: "A novel class of herpesvirus-encoded membrane-bound E3 ubiquitin ligases regulates endocytosis of proteins involved in immune recognition." in: The Journal of cell biology, Vol. 155, Issue 7, pp. 1265-73, 2001 (PubMed).

Goto, Ishido, Sato et al.: "c-MIR, a human E3 ubiquitin ligase, is a functional homolog of herpesvirus proteins MIR1 and MIR2 and has similar activity." in: The Journal of biological chemistry, Vol. 278, Issue 17, pp. 14657-68, 2003 (PubMed).

Bartee, Mansouri, Hovey Nerenberg et al.: "Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins." in: Journal of virology, Vol. 78, Issue 3, pp. 1109-20, 2004 (PubMed).

Ohmura-Hoshino, Goto, Matsuki et al.: "A novel family of membrane-bound E3 ubiquitin ligases." in: Journal of biochemistry, Vol. 140, Issue 2, pp. 147-54, 2006 (PubMed).

Validation Images
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