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Reticulon 4 (RTN4) (Isoform 1) antibody

Details for Product No. ABIN500369, Supplier: Login to see
Antigen
  • RTN4-Cw
  • DKFZp459C0314
  • NOGO
  • NOGO-A
  • reticulon-4
  • ASY
  • NI220/250
  • NOGOC
  • NSP
  • NSP-CL
  • Nbla00271
  • Nbla10545
  • Nogo-B
  • Nogo-C
  • RTN-X
  • RTN4-A
  • RTN4-B1
  • RTN4-B2
  • RTN4-C
  • 1110020G17Rik
  • AA407876
  • AA409940
  • AA960376
  • C130026I10Rik
  • NgA
  • Nogo-A
  • mKIAA0886
  • mKIAA4153
  • NI-250
  • Nogo
  • Vp20
  • r
  • rat N
  • rat NogoA
  • NGR
  • NOGOR
  • NgR
  • NgR1
  • Nogor
Alternatives
anti-Human Reticulon 4 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
Isoform 1
18
18
16
13
12
5
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
122
85
84
5
4
3
3
2
2
1
1
Host
Rabbit
127
11
5
3
2
Clonality
Polyclonal
Conjugate
Un-conjugated
5
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
112
57
49
35
22
6
6
5
3
2
1
Supplier
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Immunogen NogoA antibody was raised against a 19 amino acid peptide from near the center of human NogoA.
Isotype IgG
Specificity This antibody detects RTN4 / NOGO at Center. It is specific for NogoA and NogoE.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Alternative Name Reticulon-4 / RTN4 (RTN4 Antibody Abstract)
Background NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS, blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients. Despite its predicted molecular weight, NogoA typically migrates at 180 kDa in an SDS-PAGE. At least five isoforms of Nogo are known to exist.Synonyms: Foocen, KIAA0886, NOGO, NSP, Neurite outgrowth inhibitor, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, Reticulon-5
Gene ID 57142
NCBI Accession NP_065393
UniProt Q9NQC3
Pathways Neurotrophin Signaling Pathway
Application Notes ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Reticulon 4 (RTN4) (Isoform 1) antibody (ABIN500369) Immunohistochemistry of NogoA in mouse brain tissue with this product at 2.5 μg/ml.
Western Blotting (WB) image for anti-Reticulon 4 (RTN4) (Isoform 1) antibody (ABIN500369) Western blot analysis of NogoA in human brain tissue lysate with this product at (A) ...
Background publications Schweigreiter, Bandtlow: "Nogo in the injured spinal cord." in: Journal of neurotrauma, Vol. 23, Issue 3-4, pp. 384-96, 2006 (PubMed).

Yan, Shi, Hu et al.: "Reticulon proteins: emerging players in neurodegenerative diseases." in: Cellular and molecular life sciences : CMLS, Vol. 63, Issue 7-8, pp. 877-89, 2006 (PubMed).

Dupuis, Gonzalez de Aguilar, di Scala et al.: "Nogo provides a molecular marker for diagnosis of amyotrophic lateral sclerosis." in: Neurobiology of disease, Vol. 10, Issue 3, pp. 358-65, 2002 (PubMed).

Chen, Huber, van der Haar et al.: "Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1." in: Nature, Vol. 403, Issue 6768, pp. 434-9, 2000 (PubMed).