SKIL antibody (N-Term)

Catalog No. ABIN500786

The Rabbit Polyclonal anti-SKIL antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect SKIL in samples from Human, Mouse and Rat.

Quick Overview for SKIL antibody (N-Term) (ABIN500786)

Target: SKIL (SKI-Like Oncogene (SKIL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKIL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SKIL Antibody

Binding Specificity: N-Term

Specificity: This antibody detects SKIL / SnoN. It will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinita chromatography

Immunogen: SnoN antibody was raised against a 16 amino acid peptide from near the amino terminus of human SnoN.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for SKIL

Target: SKIL (SKI-Like Oncogene (SKIL))

Alternative Name: SKIL / SnoN

Background: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN.Synonyms: SNO, Ski-like protein, Ski-related novel protein N, Ski-related oncogene, Ski-related protein, SnoA, Snol

Gene ID: 6498

NCBI Accession: NP_005405

UniProt: P12757

Pathways: Positive Regulation of Response to DNA Damage Stimulus