SKIL antibody (N-Term)
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- Target See all SKIL Antibodies
- SKIL (SKI-Like Oncogene (SKIL))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SKIL antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody detects SKIL / SnoN. It will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).
- Cross-Reactivity (Details)
- Species reactivity (tested):Human, mouse, rat
- Purification
- Peptide affinita chromatography
- Immunogen
- SnoN antibody was raised against a 16 amino acid peptide from near the amino terminus of human SnoN.
- Isotype
- IgG
- Top Product
- Discover our top product SKIL Primary Antibody
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- Application Notes
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ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Buffer
- PBS containing 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- Target
- SKIL (SKI-Like Oncogene (SKIL))
- Alternative Name
- SKIL / SnoN (SKIL Products)
- Background
- TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN.Synonyms: SNO, Ski-like protein, Ski-related novel protein N, Ski-related oncogene, Ski-related protein, SnoA, Snol
- Gene ID
- 6498
- NCBI Accession
- NP_005405
- UniProt
- P12757
- Pathways
- Positive Regulation of Response to DNA Damage Stimulus
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