Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1) (Middle Region) antibody

Details for Product No. ABIN501657
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Antigen
Synonyms NRF1, LCR-F1, TCF11, AA408798, AW212678, Lcrf1, TCF-11
Epitope
Middle Region
(12), (8), (2), (2), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Human, Chicken
(21), (6), (2)
Host
Rabbit
(20), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(22), (11), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN501657
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human NFE2L1
Sequence FGRLRDENGR PYSPSQYALQ YAGDGSVLLI PRTMADQQAR RQERKPKDRR
Predicted Reactivity Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Guinea pig : 80 %
Characteristics This is a rabbit polyclonal antibody against NFE2L1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name NFE2L1
Background NFE2L1 activates erythroid-specific, globin gene expression.This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene, NFE2L1, and for 'nuclear respiratory factor 1' which has an official symbol of NRF1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
Molecular Weight 85 kDa
Gene ID 4779
NCBI Accession NP_003195, NM_003204
UniProt Q14494
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 772 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Background publications Wang, Kwok, Chan: "The p65 isoform of Nrf1 is a dominant negative inhibitor of ARE-mediated transcription." in: The Journal of biological chemistry, Vol. 282, Issue 34, pp. 24670-8, 2007 (PubMed).

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