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Leucine Rich Repeat Containing 50 (LRRC50) (N-Term) antibody

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Antigen
Synonyms 4930457P18Rik, Lrrc50, LRRC50, CILD13, ODA7, LLRC50, RGD1310542, lrrc50, zgc:56169
Epitope
N-Term
(7), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(20), (6), (6), (1), (1), (1), (1)
Host
Rabbit
(11), (9)
Clonality
Polyclonal
Application
Western Blotting (WB)
(19), (2), (2), (1)
Pubmed 3 references available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human LRRC50
Blocking Peptide Blocking peptide for this product available: ABIN979567
Sequence LNDTLYLHFK GFDRIENLEE YTGLRCLWLQ SNGIQKIENL EAQTELRCLF
Predicted Reactivity Dog : 92 %
Characteristics This is a rabbit polyclonal antibody against LRRC50. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name LRRC50 (LRRC50 Antibody Abstract)
Background LRRC50 contains 6 LRR (leucine-rich) repeats. It is proposed that LRRC50 to be a novel candidate gene for human cystic kidney disease, involved in regulation of microtubule-based cilia and actin-based brush border microvilli.
Molecular Weight 80 kDa
Gene ID 123872
NCBI Accession NP_848547, NM_178452
UniProt Q8NEP3
Research Area Signaling, Cytoskeleton, Microfilaments
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 725 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Leucine Rich Repeat Containing 50 (LRRC50) (N-Term) antibody (ABIN503464) WB Suggested Anti-LRRC50 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-Leucine Rich Repeat Containing 50 (LRRC50) (N-Term) antibody (ABIN503464) Host: Rabbit
Target Name: DNAAF1
Sample Tissue: Human Fetal Lung
...
Western Blotting (WB) image for anti-Leucine Rich Repeat Containing 50 (LRRC50) (N-Term) antibody (ABIN503464) Host: Rabbit
Target Name: DNAAF1
Sample Tissue: Human Fetal Muscle
Product cited in: Basten, Davis, Gillis et al.: "Mutations in LRRC50 predispose zebrafish and humans to seminomas." in: PLoS genetics, Vol. 9, Issue 4, pp. e1003384, 2013 (PubMed).

Lazrek, Goffard, Schanen et al.: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006 (PubMed).

Background publications Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Catalog No. ABIN503464
$289.00
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