LRRC50 antibody (N-Term)

Catalog No. ABIN2785360

This Rabbit Polyclonal antibody specifically detects LRRC50 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been mentioned in 3+ publications

Quick Overview for LRRC50 antibody (N-Term) (ABIN2785360)

Target: LRRC50 (Leucine Rich Repeat Containing 50 (LRRC50))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRRC50 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LRRC50 Antibody

Binding Specificity: N-Term

Purpose: LRRC50 Antibody - N-terminal region

Sequence: LNDTLYLHFK GFDRIENLEE YTGLRCLWLQ SNGIQKIENL EAQTELRCLF

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against LRRC50. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LRRC50

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP53358-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-LRRC50 antibody (ABIN2785360)

Basten, Davis, Gillis, van Rooijen, Stoop, Babala, Logister, Heath, Jonges, Katsanis, Voest, van Eeden, Medema, Ketting, Schulte-Merker, Looijenga, Giles: "Mutations in LRRC50 predispose zebrafish and humans to seminomas." in: PLoS genetics, Vol. 9, Issue 4, pp. e1003384, (2013) (PubMed).

Yang, Zhou, Ma, Wang, Chen, Meng, Yu, Zhou, Sun: "Knockdown of PPAR δ gene promotes the growth of colon cancer and reduces the sensitivity to bevacizumab in nude mice model." in: PLoS ONE, Vol. 8, Issue 4, pp. e60715, (2013) (PubMed).

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for LRRC50

Target: LRRC50 (Leucine Rich Repeat Containing 50 (LRRC50))

Alternative Name: LRRC50

Background:

Background Information: LRRC50 contains 6 LRR (leucine-rich) repeats. It is proposed that LRRC50 to be a novel candidate gene for human cystic kidney disease, involved in regulation of microtubule-based cilia and actin-based brush border microvilli.

Gene Name: Dynein, axonemal, assembly factor 1

Alternative Symbols: swt, DAU1, ODA7, CILD13, LRRC50

Protein Name: Dynein assembly factor 1, axonemal

Molecular Weight: 80kDa

Gene ID: 123872

NCBI Accession: NP_848547

UniProt: Q8NEP3