N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1) (Middle Region) antibody

Details for Product No. ABIN504351
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Antigen
Synonyms MGC82286, asah1, zgc:101637, ASAH1, AC, ACDase, ASAH, PHP, PHP32, SMAPME, Asah, 2310081N20Rik
Epitope
Middle Region
(8), (3), (3), (2), (1), (1), (1)
Reactivity
Human
(40), (25), (25), (13), (13), (12), (12), (1)
Host
Rabbit
(34), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(32), (16), (14), (10), (10), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN504351
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Immunogen Synthetic peptide directed towards the middle region of human ASAH1
Sequence QSGEGCVITR DRKESLDVYE LDAKQGRWYV VQTNYDRWKH PFFLDDRRTP
Predicted Reactivity Human : 100 %, Horse : 90 %, Mouse : 90 %, Rat : 90 %, Bovine : 80 %, Dog : 80 %
Characteristics This is a rabbit polyclonal antibody against ASAH1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name ASAH1
Background This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into
Molecular Weight 14 kDa
Gene ID 427
NCBI Accession NM_177924, NP_808592
UniProt Q13510
Research Area Signaling, Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 395 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1) (Middle Region) antibody WB Suggested Anti-ASAH1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: THP-1 cell lysate
Background publications Kim, Satta: "Population genetic analysis of the N-acylsphingosine amidohydrolase gene associated with mental activity in humans." in: Genetics, Vol. 178, Issue 3, pp. 1505-15, 2008 (PubMed).

Validation Images
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