ASAH1 antibody (AA 144-395)

Catalog No. ABIN7434806

The Rabbit Polyclonal anti-ASAH1 antibody (ABIN7434806) specifically detects ASAH1 in WB, IHC, IP and ICC. The antibody is reactive with Human samples.

Quick Overview for ASAH1 antibody (AA 144-395) (ABIN7434806)

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASAH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ASAH1 Antibody

Binding Specificity: AA 144-395

Purpose: Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 1 (ASAH1)

Specificity: The antibody is a rabbit polyclonal antibody raised against ASAH1. It has been selected for its ability to recognize ASAH1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant N-Acylsphingosine Amidohydrolase 1 (ASAH1) corresdonding to Thr144~Trp395 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ASAH1

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Alternative Name: ASAH1

Background: AC, ASAH, PHP, PHP32, Acid Ceramidase, Acylsphingosine deacylase, Putative 32 kDa heart protein

UniProt: Q13510