CD31 antibody (PE)
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- Target See all CD31 (PECAM1) Antibodies
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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Reactivity
- Human, Pig
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD31 antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Specificity
- Recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types. The extracellular region contains six Ig-like domains. Clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutant protein binding studies. Immunoprecipitates a protein of ~130 kD from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions.
- Purification
- Affinity purified
- Immunogen
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Porcine CD31/human IgGFc fusion protein.
Type of Immunogen: Fusion protein - Isotype
- IgG1
- Top Product
- Discover our top product PECAM1 Primary Antibody
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- Application Notes
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Approved: Flo (1:1)
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 100 μL of lysed whole blood or 10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Pig
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 1 mL distilled water.
- Concentration
- Lot specific
- Buffer
- Lyophilized, PBS, 0.09 % sodium azide, 5 % sucrose
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Lyophilized powder may be stored at 4°C before opening. DO NOT FREEZE! Aliquot and store at 4°C. Stable at 4°C as an undiluted liquid for 6 months. Dilute only prior to immediate use.
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- Target
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
- Alternative Name
- CAM-1 / CD31 (PECAM1 Products)
- Background
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Name/Gene ID: PECAM1
Synonyms: PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA - Gene ID
- 5175
- Pathways
- Regulation of Actin Filament Polymerization
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