Selectin P Ligand (SELPLG) antibody (APC)

Details for Product No. ABIN609651
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Antigen
Synonyms SELPLG, SELPG, CD162, CLA, PSGL-1, PSGL1, Psgl1, Selpl, Psgl-1, Selp1, 9030417L18, Slp1
Reactivity
Human
(106), (17), (14)
Host
Mouse
(70), (48), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
APC
(10), (10), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application
Flow Cytometry (FACS)
(94), (59), (40), (20), (15), (12), (10), (8), (7), (7), (2), (2), (2), (2), (2), (1), (1)
Pubmed 8 references available
Quantity 100 tests
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Catalog No. ABIN609651
421.08 $
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Immunogen Human thymocytes
Clone TC2
Isotype IgG1
Specificity The antibody TC2 reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes, it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells.
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Alternative Name SELPLG
Background CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P-, E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.
Gene ID 6404
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
General Deans, Kalt, Ledbetter et al.: "Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20." in: The Journal of biological chemistry, Vol. 270, Issue 38, pp. 22632-8, 1995 (PubMed).

Deans, Robbins, Polyak et al.: "Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 344-8, 1998 (PubMed).

Polyak, Deans: "Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence..." in: Blood, Vol. 99, Issue 9, pp. 3256-62, 2002 (PubMed).

Rose, Smith, Maloney: "Glucocorticoids and rituximab in vitro: synergistic direct antiproliferative and apoptotic effects." in: Blood, Vol. 100, Issue 5, pp. 1765-73, 2002 (PubMed).

Polyak, Ayer, Szczepek et al.: "A cholesterol-dependent CD20 epitope detected by the FMC7 antibody." in: Leukemia, Vol. 17, Issue 7, pp. 1384-9, 2003 (PubMed).

Chan, Hughes, French et al.: "CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts." in: Cancer research, Vol. 63, Issue 17, pp. 5480-9, 2003 (PubMed).

Diehl, Schmidlin, Nagasawa et al.: "STAT3-mediated up-regulation of BLIMP1 Is coordinated with BCL6 down-regulation to control human plasma cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 7, pp. 4805-15, 2008 (PubMed).

Hayden-Ledbetter, Cerveny, Espling et al.: "CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 8, pp. 2739-46, 2009 (PubMed).

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