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STRO-1 antibody

Details for Product No. ABIN638439, Supplier: Log in to see
Clonality (Clone)
Monoclonal ()
Immunocytochemistry (ICC), Flow Cytometry (FACS)
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Immunogen Human CD34 positive bone marrow cells
Clone STRO-1
Isotype IgM
Specificity The mouse monoclonal antibody STRO-1 recognizes the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
Purification Purified from cell culture supernatant by precipitation methods and ion exchange chromatography.
Purity > 95 % (by SDS-PAGE)
Background STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10 % of mononuclear cells, greater than 90 % of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
Application Notes Working concentrations should be determined by the investigator.I t is recommended that the user titrates the reagent for use in the particular testing system.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-STRO-1 antibody (ABIN638439) Surface staining of Kg1a cells with anti-STRO-1 (STRO-1) purified.
Product cited in: Kaneko, Akita, Shimauchi et al.: "Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy." in: Journal of electron microscopy, Vol. 58, Issue 6, pp. 363-73, 2009 (PubMed).

Seo, Miura, Sonoyama et al.: "Recovery of stem cells from cryopreserved periodontal ligament." in: Journal of dental research, Vol. 84, Issue 10, pp. 907-12, 2005 (PubMed).

Bensidhoum, Chapel, Francois et al.: "Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment." in: Blood, Vol. 103, Issue 9, pp. 3313-9, 2004 (PubMed).

Gronthos, Zannettino, Hay et al.: "Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow." in: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1827-35, 2003 (PubMed).

Stenderup, Justesen, Eriksen et al.: "Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 16, Issue 6, pp. 1120-9, 2001 (PubMed).

Oyajobi, Lomri, Hott et al.: "Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO-1 monoclonal antibody." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 14, Issue 3, pp. 351-61, 1999 (PubMed).

Simmons, Torok-Storb: "Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1." in: Blood, Vol. 78, Issue 1, pp. 55-62, 1991 (PubMed).