STRO-1 antibody

Details for Product No. ABIN638439, Supplier: Log in to see
Antigen
Reactivity
Human
55
Host
Mouse
55
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
5
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS)
51
25
19
3
2
2
2
Options
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Immunogen Human CD34 positive bone marrow cells
Clone STRO-1
Isotype IgM
Specificity The mouse monoclonal antibody STRO-1 recognizes the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
Purification Purified from cell culture supernatant by precipitation methods and ion exchange chromatography.
Purity > 95 % (by SDS-PAGE)
Background STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10 % of mononuclear cells, greater than 95 % of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-STRO-1 antibody (ABIN638439) Surface staining of Kg1a cells with anti-STRO-1 (STRO-1) purified.
Product cited in: Kaneko, Akita, Shimauchi, Sasano: "Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy." in: Journal of electron microscopy, Vol. 58, Issue 6, pp. 363-73, 2009 (PubMed).

Seo, Miura, Sonoyama, Coppe, Stanyon, Shi: "Recovery of stem cells from cryopreserved periodontal ligament." in: Journal of dental research, Vol. 84, Issue 10, pp. 907-12, 2005 (PubMed).

Bensidhoum, Chapel, Francois, Demarquay, Mazurier, Fouillard, Bouchet, Bertho, Gourmelon, Aigueperse, Charbord, Gorin, Thierry, Lopez: "Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment." in: Blood, Vol. 103, Issue 9, pp. 3313-9, 2004 (PubMed).

Gronthos, Zannettino, Hay, Shi, Graves, Kortesidis, Simmons: "Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow." in: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1827-35, 2003 (PubMed).

Stenderup, Justesen, Eriksen, Rattan, Kassem: "Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 16, Issue 6, pp. 1120-9, 2001 (PubMed).

Oyajobi, Lomri, Hott, Marie: "Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO-1 monoclonal antibody." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 14, Issue 3, pp. 351-61, 1999 (PubMed).

Simmons, Torok-Storb: "Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1." in: Blood, Vol. 78, Issue 1, pp. 55-62, 1991 (PubMed).