STRO-1 antibody (PE)

Catalog No. ABIN1981900

Product Details

Target: STRO-1

Reactivity: Human, Rabbit, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human CD34 positive bone marrow cells

Clone: STRO-1

Isotype: IgM lambda

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References

Kaneko, Akita, Shimauchi, Sasano: "Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy." in: Journal of electron microscopy, Vol. 58, Issue 6, pp. 363-73, (2009) (PubMed).

Seo, Miura, Sonoyama, Coppe, Stanyon, Shi: "Recovery of stem cells from cryopreserved periodontal ligament." in: Journal of dental research, Vol. 84, Issue 10, pp. 907-12, (2005) (PubMed).

Bensidhoum, Chapel, Francois, Demarquay, Mazurier, Fouillard, Bouchet, Bertho, Gourmelon, Aigueperse, Charbord, Gorin, Thierry, Lopez: "Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment." in: Blood, Vol. 103, Issue 9, pp. 3313-9, (2004) (PubMed).

Gronthos, Zannettino, Hay, Shi, Graves, Kortesidis, Simmons: "Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow." in: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1827-35, (2003) (PubMed).

Stenderup, Justesen, Eriksen, Rattan, Kassem: "Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 16, Issue 6, pp. 1120-9, (2001) (PubMed).

Oyajobi, Lomri, Hott, Marie: "Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO-1 monoclonal antibody." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 14, Issue 3, pp. 351-61, (1999) (PubMed).

Simmons, Torok-Storb: "Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1." in: Blood, Vol. 78, Issue 1, pp. 55-62, (1991) (PubMed).

Target Details

Target: STRO-1

Background: STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10 % of mononuclear cells, greater than 95 % of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.,STRO1