ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B) (AA 135-163), (Center) antibody

Details for Product No. ABIN650786
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Antigen
Synonyms ATPMB, ATPSB, hm:zehn0534, im:6793121, wu:fj38d01, zgc:111961, atpmb, atpsb, Atpsyn-beta
Epitope
AA 135-163, Center
(7), (6), (2), (2), (2)
Reactivity
Human
(35), (17), (16), (14), (13), (13), (13), (1), (1), (1), (1)
Host
Rabbit
(32), (7)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(29), (16), (14), (13), (10), (9), (6), (2)
Pubmed 3 references available
Catalog no. ABIN650786
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This ATP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 AA from the Central region of human ATP5B.
Clone RB20513
Isotype Ig
Specificity This ATP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142~171 amino acids from the Center region of human ATP5B.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATP5B
Background ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.
Synonyms: ATP synthase subunit beta, mitochondrial, ATPSB, ATPMB, ATP5B
Molecular Weight 56560 DA
Gene ID 506
UniProt P06576
Research Area Signaling, Stem Cells, Protein Modifications
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.398 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B) (AA 135-163), (Center) antibody Western blot analysis of ATP5B Antibody (Center) (ABIN650786) in WiDr cell line lysates (35 µg/lane).ATP5B (arrow) was detected using the purified polyclonal antibody.
anti-ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B) (AA 135-163), (Center) antibody (2) Western blot analysis of ATP5B (arrow) using rabbit polyclonal ATP5B Antibody (Center) (ABIN650786). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2) .
anti-ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B) (AA 135-163), (Center) antibody (3) Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Neckelmann, Warner, Chung et al.: "The human ATP synthase beta subunit gene: sequence analysis, chromosome assignment, and differential expression." in: Genomics, Vol. 5, Issue 4, pp. 829-43, 1990 (PubMed).

Wallace, Ye, Neckelmann et al.: "Sequence analysis of cDNAs for the human and bovine ATP synthase beta subunit: mitochondrial DNA genes sustain seventeen times more mutations." in: Current genetics, Vol. 12, Issue 2, pp. 81-90, 1988 (PubMed).

Ohta, Tomura, Matsuda et al.: "Gene structure of the human mitochondrial adenosine triphosphate synthase beta subunit." in: The Journal of biological chemistry, Vol. 263, Issue 23, pp. 11257-62, 1988 (PubMed).

Hosts (32), (7)
Reactivities (35), (17), (16), (14), (13), (13), (13), (1), (1), (1), (1)
Applications (29), (16), (14), (13), (10), (9), (6), (2)
Conjugates (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (6), (2), (2), (2)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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