ATP5B antibody (Internal Region)

Catalog No. ABIN6260141

Product Details anti-ATP5B Antibody

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ATPB Antibody detects endogenous levels of total ATPB.

Predicted Reactivity: Pig,Bovine,Horse,Rabbit,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATPB, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATP5B

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Alternative Name: ATP5B (ATP5B Products)

Synonyms: atpmb antibody, atpsb antibody, Atpsyn-beta antibody, hm:zehn0534 antibody, im:6793121 antibody, wu:fj38d01 antibody, zgc:111961 antibody, ATPMB antibody, ATPSB antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide antibody, ATP synthase subunit beta, mitochondrial antibody, ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide S homeolog antibody, ATP5B antibody, atp5b antibody, Atp5b antibody, LOC100401662 antibody, LOC100635763 antibody, atp5b.S antibody

Background:

Description: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Gene: ATP5B

Molecular Weight: 45-57 kDa

Gene ID: 506

UniProt: P06576

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process