Histone H1.5 (N-Term), (AA 33-60) antibody

Details for Product No. ABIN651424
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Synonyms H1, H1.5, H1B, H1F5, H1s-3, H1f5
N-Term, AA 33-60
(5), (1), (1)
Clonality (Clone)
Polyclonal ()
Western Blotting (WB), Flow Cytometry (FACS)
(7), (5), (4), (4), (2)
Pubmed 2 references available
Catalog no. ABIN651424
Quantity 400 µL
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Immunogen This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 AA from the N-terminal region of human HIST1H1B.
Clone RB30034
Isotype Ig
Specificity This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the N-terminal region of human HIST1H1B.
Predicted Reactivity Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name HIST1H1B
Background Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family.
Synonyms: Histone H1a, Histone H1.5,H1F5, HIST1H1B
Molecular Weight 22580 DA
Gene ID 3009
UniProt P16401
Research Area Cancer
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Histone H1.5 (N-Term), (AA 33-60) antibody Western blot analysis of HIST1H1B Antibody (N-term) (ABIN651424) in NCI-H460 cell line lysates (35 µg/lane).HIST1H1B (arrow) was detected using the purified polyclonal antibody.
anti-Histone H1.5 (N-Term), (AA 33-60) antibody (2) HIST1H1B Antibody (N-term) (ABIN651424) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Trojer, Li, Sims et al.: "L3MBTL1, a histone-methylation-dependent chromatin lock." in: Cell, Vol. 129, Issue 5, pp. 915-28, 2007 (PubMed).

Happel, Stoldt, Schmidt et al.: "M phase-specific phosphorylation of histone H1.5 at threonine 10 by GSK-3." in: Journal of molecular biology, Vol. 386, Issue 2, pp. 339-50, 2009 (PubMed).

Hosts (7)
Reactivities (7)
Applications (7), (5), (4), (4), (2)
Epitopes (5), (1), (1)
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