Histone H1.5 antibody (pSer17)

Catalog No. ABIN6971747

Product Details anti-Histone H1.5 Antibody

Target: Histone H1.5 (HIST1H1B)

Binding Specificity: pSer17

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H1.5 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Characteristics: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H1.5 is an H1 isotype expressed in somatic cells and is heavily phosphorylated during mitosis. Phosphorylation of H1.5 at serine 17 appears in prometaphase and disappears in telophase. The hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin is maximally condensed. Phosphorylation of H1.5 at serine 17 can be catalyzed by GSK-3. Histone H1.5S17ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogen: This Histone H1.5 phospho Ser17 antibody was raised against a peptide containing phospho Ser17 of human histone H1.5.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Histone H1.5

Target: Histone H1.5 (HIST1H1B)

Alternative Name: Histone H1.5 (HIST1H1B Products)

Synonyms: H1 antibody, H1.5 antibody, H1B antibody, H1F5 antibody, H1s-3 antibody, H1f5 antibody, histone cluster 1, H1b antibody, histone cluster 1 H1 family member b antibody, HIST1H1B antibody, Hist1h1b antibody

Molecular Weight: 36 kDa

NCBI Accession: NP_005313