Carbonic Anhydrase VB, Mitochondrial (CA5B) (C-Term), (AA 253-283) antibody

Details for Product No. ABIN652315
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Antigen
Synonyms CA5B, zgc:171653, 7330410H16Rik, CAVB, Ca5b, CarVb, D730005F19Rik, CA-VB
Epitope
C-Term, AA 253-283
(7), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(34), (20), (19)
Host
Rabbit
(40), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(6), (3), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(42), (32), (14), (13), (12), (8), (6), (2), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN652315
291.50 $
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Immunogen This CA5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-283 AA from the C-terminal region of human CA5B.
Clone RB18377
Isotype Ig
Specificity This CA5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261~290 amino acids from the C-terminal region of human CA5B.
Predicted Reactivity Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name CA5B
Background CA5B belongs a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. This protein is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. The protein has a wider tissue distribution than CA VA, which is restricted to the liver.
Synonyms: Carbonic anhydrase 5B, mitochondrial, Carbonic anhydrase VB, CA-VB, Carbonate dehydratase VB, CA5B
Molecular Weight 36434 DA
Gene ID 11238
UniProt Q9Y2D0
Research Area Cancer, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Carbonic Anhydrase VB, Mitochondrial (CA5B) (C-Term), (AA 253-283) antibody Western blot analysis of CA5B Antibody (C-term) (ABIN652315) in mouse liver tissue lysates (35 µg/lane). CA5B (arrow) was detected using the purified polyclonal antibody.
anti-Carbonic Anhydrase VB, Mitochondrial (CA5B) (C-Term), (AA 253-283) antibody (2) CA5B Antibody (C-term) (ABIN652315) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-Carbonic Anhydrase VB, Mitochondrial (CA5B) (C-Term), (AA 253-283) antibody (3) CA5B Antibody (C-term) (ABIN652315) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Fujikawa-Adachi, Nishimori, Taguchi et al.: "Human mitochondrial carbonic anhydrase VB. cDNA cloning, mRNA expression, subcellular localization, and mapping to chromosome x." in: The Journal of biological chemistry, Vol. 274, Issue 30, pp. 21228-33, 1999 (PubMed).

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