Hemopoietic Cell Kinase (HCK) (N-Term), (AA 131-156) antibody

Details for Product No. ABIN652384
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Antigen
Synonyms laloo, HCK, MGC107870, JTK9, p59Hck, p61Hck, AI849071, Bmk, Hctk, p56Hck
Epitope
N-Term, AA 131-156
(23), (7), (7), (5), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(87), (34), (31), (12), (12)
Host
Rabbit
(71), (17), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(69), (49), (25), (10), (9), (9), (8), (7), (4), (2), (2)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN652384
238.50 $
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Immunogen This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-156 AA from the N-terminal region of human HCK.
Clone RB20667
Isotype Ig
Specificity This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the N-terminal region of human HCK.
Predicted Reactivity Mouse (Murine),Monkey,Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name HCK
Background HCK is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq].
Synonyms: Tyrosine-protein kinase HCK,HCK,
Molecular Weight 59600 DA
Gene ID 3055
NCBI Accession NP_002101
UniProt P08631
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Hemopoietic Cell Kinase (HCK) (N-Term), (AA 131-156) antibody HCK Antibody (N-term) (ABIN652384) western blot analysis in mouse lung tissue lysates (15 µg/lane).This demonstrates the HCK antibody detected HCK protein (arrow).
anti-Hemopoietic Cell Kinase (HCK) (N-Term), (AA 131-156) antibody (2) HCK Antibody (N-term) (ABIN652384) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-Hemopoietic Cell Kinase (HCK) (N-Term), (AA 131-156) antibody (3) HCK Antibody (N-term) (ABIN652384) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. ..." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Kennah, Ringrose, Zhou et al.: "Identification of tyrosine kinase, HCK, and tumor suppressor, BIN1, as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells." in: Blood, Vol. 113, Issue 19, pp. 4646-55, 2009 (PubMed).

Hassan, Suzu, Hiyoshi et al.: "Dys-regulated activation of a Src tyroine kinase Hck at the Golgi disturbs N-glycosylation of a cytokine receptor Fms." in: Journal of cellular physiology, Vol. 221, Issue 2, pp. 458-68, 2009 (PubMed).

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