Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1) (AA 252-281), (Center) antibody

Details for Product No. ABIN652732
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Antigen
Synonyms CP7B, CBAS3, SPG5A, hct-1, AW261589, D3Ertd552e, CYP7B1
Epitope
AA 252-281, Center
(4), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(40), (13), (13), (12), (1)
Host
Rabbit
(28), (10), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
(30), (16), (10), (8), (3), (2), (2), (2)
Pubmed 1 reference available
Catalog no. ABIN652732
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
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Immunogen This CYP7B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-281 AA from the Central region of human CYP7B1.
Clone RB17087
Isotype Ig
Specificity This CYP7B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259~289 amino acids from the Center region of human CYP7B1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name CYP7B1
Background CYP7B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.
Synonyms: Cytochrome P450 7B1, 25-hydroxycholesterol 7-alpha-hydroxylase, Oxysterol 7-alpha-hydroxylase, CYP7B1
Pathways: Steroid hormone biosynthesis, Primary bile acid biosynthesis
Molecular Weight 58256 Da
Gene ID 9420
UniProt O75881
Research Area Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1) (AA 252-281), (Center) antibody Western blot analysis of CYP7B1 Antibody (Center) (ABIN652732) in Hela cell line lysates (35 µg/lane). CYP7B1 (arrow) was detected using the purified polyclonal antibody.
anti-Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1) (AA 252-281), (Center) antibody (2) Formalin-fixed and paraffin-embedded human brain tissue reacted with CALR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1) (AA 252-281), (Center) antibody (3) CYP7B1 Antibody (Center) (ABIN652732) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Schwarz, Lund, Russell: "Two 7 alpha-hydroxylase enzymes in bile acid biosynthesis." in: Current opinion in lipidology, Vol. 9, Issue 2, pp. 113-8, 1998 (PubMed).

Hosts (28), (10), (3)
Reactivities (40), (13), (13), (12), (1)
Applications (30), (16), (10), (8), (3), (2), (2), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (2), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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