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Alkaline Ceramidase 3 (ACER3) (C-Term), (AA 224-250) antibody

Details for Product No. ABIN652900
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Antigen
Synonyms phca, PHCA, APHC, 1110057L18Rik, 5430429L08Rik, AV015045, Phca, RGD1561254
Epitope
C-Term, AA 224-250
(7), (6), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(22), (12)
Host
Rabbit
(22)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(22), (19), (12), (9), (3), (2), (1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN652900
291.50 $
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Immunogen This ACER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-250 AA from the C-terminal region of human ACER3.
Clone RB22389
Isotype Ig
Specificity This ACER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230~259 amino acids from the C-terminal region of human ACER3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ACER3
Background ACER3 hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.
Synonyms: Alkaline dihydroceramidase SB89, Alkaline phytoceramidase, aPHC, Alkaline ceramidase 3, Alkaline CDase 3, AlkCDase 3, ACER3, APHC, PHCA
Molecular Weight 31566 DA
Gene ID 55331
UniProt Q9NUN7
Research Area Cancer, Cell Structure
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Alkaline Ceramidase 3 (ACER3) (C-Term), (AA 224-250) antibody Western blot analysis of ACER3 Antibody (C-term) (ABIN652900) in HepG2 cell line lysates (35 µg/lane). ACER3 (arrow) was detected using the purified polyclonal antibody.
anti-Alkaline Ceramidase 3 (ACER3) (C-Term), (AA 224-250) antibody (2) ACER3 Antibody (C-term) (ABIN652900) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Mao, Obeid: "Ceramidases: regulators of cellular responses mediated by ceramide, sphingosine, and sphingosine-1-phosphate." in: Biochimica et biophysica acta, Vol. 1781, Issue 9, pp. 424-34, 2008 (PubMed).

Wheeler, Metter, Tanaka et al.: "Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000685, 2009 (PubMed).

Validation Images
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