Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 226-253), (Center) antibody

Details for Product No. ABIN652928
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Antigen
Synonyms rama1, C13orf3, RAMA1, F630043A04Rik, RGD1307201, C12H13ORF3, fc23d11, si:rp71-68n21.13, wu:fc23d11
Epitope
AA 226-253, Center
(12), (9), (2), (1), (1), (1), (1)
Reactivity
Human
(40), (21), (21), (11), (11), (2)
Host
Rabbit
(35), (4), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(27), (20), (9), (9), (3), (2), (2), (1)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN652928
291.50 $
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Immunogen This C13orf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-253 AA from the Central region of human C13orf3.
Clone RB23058
Isotype Ig
Specificity This C13orf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232~262 amino acids from the Center region of human C13orf3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name C13orf3
Background Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.
Synonyms: Spindle and kinetochore-associated protein 3, SKA3, C13orf3, RAMA1
Molecular Weight 46359 DA
Gene ID 221150
UniProt Q8IX90
Research Area Cancer, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.36 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 226-253), (Center) antibody Western blot analysis of C13orf3 Antibody (Center) (ABIN652928) in Hela, 293 cell line lysates (35 µg/lane). C13orf3 (arrow) was detected using the purified polyclonal antibody.
anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 226-253), (Center) antibody (2) C13orf3 Antibody (Center) (ABIN652928) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 226-253), (Center) antibody (3) C13orf3 Antibody (Center) (ABIN652928) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Validation Images
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