Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1) (C-Term), (AA 482-508) antibody

Details for Product No. ABIN652999
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Antigen
Synonyms cp2b, cyp1, cyp1alpha, cyp27b, p450c1, pddr, vdd1, vddr, vddri, vdr, CP2B, CYP1, CYP1alpha, CYP27B, P450c1, PDDR, VDD1, VDDR, VDDRI, VDR, Cyp40, Cp2b, Cyp1, Cyp27b, Pddr, Vdd1, Vddr, VddrI, Vdr
Epitope
C-Term, AA 482-508
(11), (7), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(16), (2), (1)
Host
Rabbit
(18), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(19), (11), (10), (8), (5), (5), (4), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN652999
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Immunogen This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-508 AA from the C-terminal region of human CYP27B1.
Clone RB16978
Isotype Ig
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name CYP27B1
Background CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Synonyms: Cytochrome P450VD1-alpha, Cytochrome P450C1 alpha, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, VD3 1A hydroxylase, 25-OHD-1 alpha-hydroxylase, Calcidiol 1-monooxygenase, Cytochrome p450 27B1, Cytochrome P450 subfamily XXVIIB polypeptide 1, 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, CYP27B, CYP1ALPHA, CYP27B1
Pathways: Tuberculosis
Molecular Weight 56504 Da
Gene ID 1594
UniProt O15528
Research Area Cell Structure
Application Notes Suggested dilutions:
WB = 1:1000, IHC = 1:10-50, FACS = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1) (C-Term), (AA 482-508) antibody Western blot analysis of CYP27B1 Antibody (C-term) (ABIN652999) in mouse kidney tissue lysates (35 µg/lane). CYP27B1 (arrow) was detected using the purified polyclonal antibody.
anti-Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1) (C-Term), (AA 482-508) antibody (2) CYP27B1 Antibody (C-term) (ABIN652999) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1) (C-Term), (AA 482-508) antibody (3) CYP27B1 Antibody (C-term) (ABIN652999)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Product cited in: Mulligan, Nagel, OConnell et al.: "Cigarette smoke exposure is associated with vitamin D3 deficiencies in patients with chronic rhinosinusitis." in: The Journal of allergy and clinical immunology, Vol. 134, Issue 2, pp. 342-349.e1, 2014 (PubMed).

Background publications Simon, Munger, Xing Yang et al.: "Polymorphisms in vitamin D metabolism related genes and risk of multiple sclerosis." in: Multiple sclerosis (Houndmills, Basingstoke, England), Vol. 16, Issue 2, pp. 133-8, 2010 (PubMed).

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