CYP27B1 antibody (C-Term)

Catalog No. ABIN3042787

This Rabbit Polyclonal antibody specifically detects CYP27B1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CYP27B1 antibody (C-Term) (ABIN3042787)

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP27B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CYP27B1 Antibody

Binding Specificity: AA 475-508, C-Term

Purpose: Anti-CYP27B1 Antibody

Sequence: HFEVQPEPGA APVRPKTRTV LVPERSINLQ FLDR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CYP27B1 Antibody (ABIN3042787). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CYP27B1, different from the related mouse and rat sequences by six amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CYP27B1

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Alternative Name: CYP27B1

Background:

Synonyms: 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial,1.14.13.13,25-OHD-1 alpha-hydroxylase,25-hydroxyvitamin D (3) 1-alpha-hydroxylase,VD3 1A hydroxylase,Calcidiol 1-monooxygenase,Cytochrome P450 subfamily XXVIIB polypeptide 1,Cytochrome P450C1 alpha,Cytochrome P450VD1-alpha,Cytochrome p450 27B1,CYP27B1,CYP1ALPHA, CYP27B,

Tissue Specificity: Kidney.

Background: CYP27B1 belongs to the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Sequence Similarities: Belongs to the cytochrome P450 family.

Molecular Weight: 57 kDa

UniProt: O15528

Pathways: Metabolism of Steroid Hormones and Vitamin D