K2C78 (AA 176-203), (Center) antibody

Details for Product No. ABIN653813
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Antigen
Epitope
AA 176-203, Center
(1), (1)
Reactivity
Human
(1)
Host
Rabbit
(1)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Flow Cytometry (FACS)
(1), (1), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN653813
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Immunogen This K2C78 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-203 AA from the Central region of human K2C78.
Clone RB24916
Isotype Ig
Specificity This K2C78 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182~212 amino acids from the Central region of human K2C78.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background This gene is a member of the type II keratin gene family and encodes a protein with an intermediate filament domain. Keratins are the major structural proteins in epithelial cells, forming a cytoplasmic network of 10 to 12 nm wide intermediate filaments and creating a scaffold that gives cells the ability to withstand mechanical and non-mechanical stresses. The genes of the type II keratin family are located as a gene cluster at 12p13.13. Four pseudogenes of this gene family have been identified.
Synonyms: Keratin-5b, Type-II keratin Kb40, Keratin-78, K78, Cytokeratin-78, CK-78, Keratin, type II cytoskeletal 78, KB40, K5B, KRT78
Molecular Weight 56866 DA
Gene ID 196374
UniProt Q8N1N4
Research Area Cell Structure
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-K2C78 (AA 176-203), (Center) antibody Western blot analysis of K2C78 Antibody (Center) (ABIN653813) in Hela cell line lysates (35 µg/lane). K2C78 (arrow) was detected using the purified polyclonal antibody.
anti-K2C78 (AA 176-203), (Center) antibody (2) K2C78 Antibody (Center) (ABIN653813) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Schweizer, Bowden, Coulombe et al.: "New consensus nomenclature for mammalian keratins." in: The Journal of cell biology, Vol. 174, Issue 2, pp. 169-74, 2006 (PubMed).

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