Transcription Factor A, Mitochondrial (TFAM) (C-Term), (AA 216-246) antibody

Details for Product No. ABIN654229
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Antigen
Synonyms tcf6, mttf1, mttfa, tcf6l1, tcf6l2, tcf6l3, mttfa-A, xl-mtTFA, MGC153358, zgc:153358, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3, AI661103, Hmgts, mtTFA, tsHMG, Mttfa
Epitope
C-Term, AA 216-246
(12), (8), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(61), (21), (20), (14), (13), (13)
Host
Rabbit
(68), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(46), (27), (22), (19), (19), (16), (10), (9), (4), (3), (3), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN654229
238.50 $
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Immunogen This TFAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 AA from the C-terminal region of human TFAM.
Clone RB27631
Isotype Ig
Specificity This TFAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-254 amino acids from the C-terminal region of human TFAM.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name TFAM
Background This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.
Synonyms: Transcription factor A, mitochondrial,TFAM,TCF6, TCF6L2
Molecular Weight 29097 DA
Gene ID 7019
NCBI Accession NP_003192
UniProt Q00059
Research Area Translation Factors, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Application Notes IHC = 1:50-100, FACS = 1:10-50, IF = 1:10-50, WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Transcription Factor A, Mitochondrial (TFAM) (C-Term), (AA 216-246) antibody Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody (C-term). NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TFAM immunoreactivity is localized to mitochondrion significantly.
anti-Transcription Factor A, Mitochondrial (TFAM) (C-Term), (AA 216-246) antibody (2) TFAM antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TFAM antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Transcription Factor A, Mitochondrial (TFAM) (C-Term), (AA 216-246) antibody (3) TFAM Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-Transcription Factor A, Mitochondrial (TFAM) (C-Term), (AA 216-246) antibody (4) TFAM Antibody (C-term) western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the TFAM antibody detected the TFAM protein (arrow).
Background publications Shulman, Chibnik, Aubin et al.: "Intermediate phenotypes identify divergent pathways to Alzheimer's disease." in: PLoS ONE, Vol. 5, Issue 6, pp. e11244, 2010 (PubMed).

Validation Images
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