TFAM antibody

Catalog No. ABIN2855585

Product Details anti-TFAM Antibody

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFAM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to mtTFA (transcription factor A, mitochondrial)
mtTFA antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human mtTFA. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.23 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TFAM

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Alternative Name: transcription factor A, mitochondrial (TFAM Products)

Synonyms: tcf6 antibody, mttf1 antibody, mttfa antibody, tcf6l1 antibody, tcf6l2 antibody, tcf6l3 antibody, mttfa-A antibody, xl-mtTFA antibody, MGC153358 antibody, zgc:153358 antibody, MTTF1 antibody, MTTFA antibody, TCF6 antibody, TCF6L1 antibody, TCF6L2 antibody, TCF6L3 antibody, AI661103 antibody, Hmgts antibody, mtTFA antibody, tsHMG antibody, Mttfa antibody, transcription factor A, mitochondrial antibody, transcription factor A, mitochondrial L homeolog antibody, TFAM antibody, tfam.L antibody, tfam antibody, Tfam antibody

Background: This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.

Cellular Localization: Mitochondrion

Molecular Weight: 29 kDa

Gene ID: 7019

UniProt: Q00059

Pathways: Chromatin Binding