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Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 399-427), (Center) antibody

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Synonyms SREBF2, srebp2, SREBP2, zgc:158371, AI608257, SREBP-2, SREBP2gc, bHLHd2, lop13, nuc, Srebf2_retired
AA 399-427, Center
(17), (16), (12), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(61), (19), (18), (3), (3), (3), (2), (1), (1)
(55), (7), (1)
Clonality (Clone)
Polyclonal ()
(3), (2), (2), (2), (2), (2), (1)
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(55), (28), (27), (14), (11), (6), (5), (2), (2), (1), (1)
Pubmed 3 references available
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Quantity 400 μL
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Immunogen This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-427 AA from the Central region of human SREBF2.
Clone RB24513
Isotype Ig
Specificity This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-431 amino acids from the Central region of human SREBF2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SREBF2 (SREBF2 Antibody Abstract)
Background SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol. LS-B1609 polyclonal antibody detects both precursor and active forms of the protein in tissues and cells such as liver, brown fat, testis, Hep-G2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.
Synonyms: Sterol regulatory element-binding protein 2,SREBF2,BHLHD2, SREBP2
Molecular Weight 123688 DA
Gene ID 6721
NCBI Accession NP_004590
UniProt Q12772
Research Area Translation Factors, Phospho-specific antibodies, Cell Structure, Protein Modifications, Transcription Factors, Signaling, Metabolism
Application Notes IF = 1:10-50, WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Immunofluorescence (IF) image for anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 399-427), (Center) antibody (ABIN654630) Confocal immunofluorescent analysis of SREBF2 Antibody (Center) (ABIN654630) with Hep...
Background publications Gerin, Clerbaux, Haumont et al.: "Expression of miR-33 from an SREBP2 intron inhibits cholesterol export and fatty acid oxidation." in: The Journal of biological chemistry, Vol. 285, Issue 44, pp. 33652-61, 2010 (PubMed).

Marquart, Allen, Ory et al.: "miR-33 links SREBP-2 induction to repression of sterol transporters." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 27, pp. 12228-32, 2010 (PubMed).

Najafi-Shoushtari, Kristo, Li et al.: "MicroRNA-33 and the SREBP host genes cooperate to control cholesterol homeostasis." in: Science (New York, N.Y.), Vol. 328, Issue 5985, pp. 1566-9, 2010 (PubMed).

Catalog No. ABIN654630
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