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Details for Product No. ABIN654630

Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 399-427), (Center) antibody

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Antigen
Synonyms SREBP-2, SREBP2, bHLHd2, AI608257, SREBP2gc, lop13, nuc, Srebf2_retired, SREBF2, srebp2, zgc:158371
Epitope
»Alternatives AA 399-427, Center
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality (Clone) Polyclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 3 references available
Catalog no. ABIN654630
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-427 AA from the Central region of human SREBF2.
Clone RB24513
Isotype Ig
Specificity This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-431 amino acids from the Central region of human SREBF2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SREBF2
Background SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol. LS-B1609 polyclonal antibody detects both precursor and active forms of the protein in tissues and cells such as liver, brown fat, testis, Hep-G2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.
Synonyms: Sterol regulatory element-binding protein 2,SREBF2,BHLHD2, SREBP2
Molecular Weight 123688 DA
Gene ID 6721
UniProt NP_004590.2, Q12772
Research Area Translation Factors, Phospho-specific antibodies, Cell Structure, Protein Modifications, Transcription Factors, Signaling, Metabolism
Application Notes Recommend dilutions: IF : 1:10-50,WB : 1:1000,IHC : 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Background publications Najafi-Shoushtari, Kristo, Li et al.: "MicroRNA-33 and the SREBP host genes cooperate to control cholesterol homeostasis." in: Science (New York, N.Y.), Vol. 328, Issue 5985, pp. 1566-9, 2010 (PubMed).

Marquart, Allen, Ory et al.: "miR-33 links SREBP-2 induction to repression of sterol transporters." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 27, pp. 12228-32, 2010 (PubMed).

Gerin, Clerbaux, Haumont et al.: "Expression of miR-33 from an SREBP2 intron inhibits cholesterol export and fatty acid oxidation." in: The Journal of biological chemistry, Vol. 285, Issue 44, pp. 33652-61, 2010 (PubMed).

Alternatives for antigen "Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2)", type "Antibodies"
Hosts (30), (2)
Reactivities (31), (18), (17), (14), (2), (2), (1), (1)
Applications (23), (10), (9), (8), (5), (4), (4)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3), (2), (2), (1), (1), (1), (1), (1), (1)
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