UPF2 Regulator of Nonsense Transcripts Homolog (Yeast) (UPF2) (AA 630-656), (Center) antibody

Details for Product No. ABIN654649
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Antigen
Epitope
AA 630-656, Center
(8), (2), (2), (1), (1), (1)
Reactivity
Human
(15), (2), (1), (1), (1)
Host
Rabbit
(13), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(13), (10), (8), (1), (1), (1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN654649
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Immunogen This UPF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 630-656 AA from the Central region of human UPF2.
Clone RB21963
Isotype Ig
Specificity This UPF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 633-660 amino acids from the Central region of human UPF2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name UPF2
Background UPF2 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p.
Synonyms: Regulator of nonsense transcripts 2,UPF2,KIAA1408, RENT2
Molecular Weight 147810 DA
Gene ID 26019
NCBI Accession NP_056357
UniProt Q9HAU5
Research Area Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Signaling, Cell Structure
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-UPF2 Regulator of Nonsense Transcripts Homolog (Yeast) (UPF2) (AA 630-656), (Center) antibody UPF2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the UPF2 antibody detected the UPF2 protein (arrow).
anti-UPF2 Regulator of Nonsense Transcripts Homolog (Yeast) (UPF2) (AA 630-656), (Center) antibody (2) UPF2 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Cronin, Tomik, Bradley et al.: "Screening for replication of genome-wide SNP associations in sporadic ALS." in: European journal of human genetics : EJHG, Vol. 17, Issue 2, pp. 213-8, 2009 (PubMed).

Clerici, Mourão, Gutsche et al.: "Unusual bipartite mode of interaction between the nonsense-mediated decay factors, UPF1 and UPF2." in: The EMBO journal, Vol. 28, Issue 15, pp. 2293-306, 2010 (PubMed).

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