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Denticleless E3 Ubiquitin Protein Ligase Homolog (Drosophila) (DTL) (AA 229-256), (Center) antibody

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Synonyms CDT2, DCAF2, L2DTL, RAMP, RGD1310439, 2810047L02Rik, 5730564G15Rik, Ramp
AA 229-256, Center
(17), (13), (8), (4), (4), (2), (2), (1), (1), (1)
Human, Mouse (Murine)
(41), (12), (8), (1), (1)
(40), (1)
Clonality (Clone)
Polyclonal ()
(2), (2), (2), (2), (2), (2), (1)
Flow Cytometry (FACS), Western Blotting (WB)
(34), (19), (15), (5), (4), (4), (2), (1), (1)
Pubmed 3 references available
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Quantity 400 μL
Shipping to United States ( )
Availability Will be delivered in 2 to 4 Business Days
Immunogen This DTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 AA from the Central region of human DTL.
Clone RB24420
Isotype Ig
Specificity This DTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of human DTL.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name DTL (DTL Antibody Abstract)
Background Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
Synonyms: Denticleless protein homolog,DTL,CDT2, CDW1, DCAF2, L2DTL, RAMP
Molecular Weight 79468 DA
Gene ID 51514
NCBI Accession NP_057532
UniProt Q9NZJ0
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Structure, Protein Modifications, Chromatin, Signaling
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Flow Cytometry (FACS) image for anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (Drosophila) (DTL) (AA 229-256), (Center) antibody (ABIN654926) DTL Antibody (Center) (ABIN654926) flow cytometric analysis of Hela cells (right hist...
Background publications Centore, Havens, Manning et al.: "CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase." in: Molecular cell, Vol. 40, Issue 1, pp. 22-33, 2010 (PubMed).

Abbas, Shibata, Park et al.: "CRL4(Cdt2) regulates cell proliferation and histone gene expression by targeting PR-Set7/Set8 for degradation." in: Molecular cell, Vol. 40, Issue 1, pp. 9-21, 2010 (PubMed).

Abbas, Sivaprasad, Terai et al.: "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex." in: Genes & development, Vol. 22, Issue 18, pp. 2496-506, 2008 (PubMed).

Catalog No. ABIN654926
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