Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 108-138), (Center) antibody

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Antigen
Synonyms Lin28, csdd1, lin-28, lin28, zcchc1, LIN28, CSDD1, LIN-28, ZCCHC1, lin-28A, AL024421, ENSMUSG00000070700, Gm10299, Lin-28, Tex17, RGD1566408, sb:cb650, zgc:55584
Epitope
AA 108-138, Center
(13), (7), (6), (5), (4), (4), (3), (2), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(89), (29), (22), (13), (13), (1)
Host
Rabbit
(63), (29)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(74), (35), (23), (22), (20), (16), (11), (10), (9), (6), (6), (4), (3)
Pubmed 5 references available
Quantity 400 μL
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Catalog No. ABIN655156
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Immunogen This LIN28A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 AA from the Central region of human LIN28A.
Clone RB14827
Isotype Ig
Specificity This LIN28A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-147 amino acids from the Central region of human LIN28A.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name LIN28A
Background Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Synonyms: LIN28A,Protein lin-28 homolog A,CSDD1, LIN28, ZCCHC1
Molecular Weight 22743 Da
Gene ID 79727
UniProt Q9H9Z2
Research Area Cell Structure, Signaling, Chromatin
Application Notes IF = 1:10-50, WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 108-138), (Center) antibody LIN28A Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LIN28A antibody detected the LIN28A protein (arrow).
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 108-138), (Center) antibody (2) Fluorescent confocal image of SY5Y cells stained with AP11206c LIN28A (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP11206c LIN28A (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Lin28a immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 108-138), (Center) antibody (3) LIN28A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LIN28A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 108-138), (Center) antibody (4) LIN28A Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications West, Viswanathan, Yabuuchi et al.: "A role for Lin28 in primordial germ-cell development and germ-cell malignancy." in: Nature, Vol. 460, Issue 7257, pp. 909-13, 2009 (PubMed).

Heo, Joo, Kim et al.: "TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation." in: Cell, Vol. 138, Issue 4, pp. 696-708, 2009 (PubMed).

Iliopoulos, Hirsch, Struhl: "An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation." in: Cell, Vol. 139, Issue 4, pp. 693-706, 2009 (PubMed).

Qiu, Ma, Wang et al.: "Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells." in: Nucleic acids research, Vol. 38, Issue 4, pp. 1240-8, 2010 (PubMed).

Peng, Maihle, Huang: "Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer." in: Oncogene, Vol. 29, Issue 14, pp. 2153-9, 2010 (PubMed).

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