Pinin, Desmosome Associated Protein (PNN) (AA 209-239), (Center) antibody

Details for Product No. ABIN655502
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Antigen
Synonyms PNN, MGC127754, pnn, MGC88923, Pinin, DKFZp459I0427, CG8383, Dmel\\CG8383, dPnn, AU045199, D12Ertd512e, DRS, DRSP, SDK3, memA, wu:fb24d03
Epitope
AA 209-239, Center
(14), (8), (7), (6), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(59), (32), (24), (18), (14), (13), (13), (1), (1)
Host
Rabbit
(50), (10)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(51), (31), (10), (10), (7), (6), (5), (2), (1)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN655502
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Immunogen This PNN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-239 AA from the Central region of human PNN.
Clone RB17516
Isotype Ig
Specificity This PNN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-248 amino acids from the Central region of human PNN.
Predicted Reactivity Cow (Bovine),Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name PNN
Background Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene, the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.
Synonyms: Pinin,PNN,DRS, MEMA
Molecular Weight 81614 Da
Gene ID 5411
UniProt Q9H307
Research Area Cell Structure, Signaling, Protein Modifications, Cancer, Phospho-specific antibodies, Transcription Factors, Translation Factors
Application Notes WB = 1:1000, IHC = 1:10-50, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.34 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Pinin, Desmosome Associated Protein (PNN) (AA 209-239), (Center) antibody Confocal immunofluorescent analysis of PNN Antibody (Center) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
anti-Pinin, Desmosome Associated Protein (PNN) (AA 209-239), (Center) antibody (2) PNN Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PNN antibody detected the PNN protein (arrow).
anti-Pinin, Desmosome Associated Protein (PNN) (AA 209-239), (Center) antibody (3) PNN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PNN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Background publications Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Alpatov, Shi, Munguba et al.: "Corepressor CtBP and nuclear speckle protein Pnn/DRS differentially modulate transcription and splicing of the E-cadherin gene." in: Molecular and cellular biology, Vol. 28, Issue 5, pp. 1584-95, 2008 (PubMed).

Validation Images
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