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Details for Product No. ABIN657824

Splicing Factor Proline/glutamine-Ric (SFPQ) (C-Term), (AA 676-705) antibody

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Antigen
Synonyms 1110004P21Rik, 2810416M14Rik, 5730453G22Rik, 9030402K04Rik, AU021830, D4Ertd314e, Gm12940, OTTMUSG00000009329, PSF, REP1, POMP100, hm:zeh0027, wu:fa11h12, wu:fd10f03, zgc:85935
Epitope
»Alternatives C-Term, AA 676-705
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality (Clone) Polyclonal ()
Application
»Alternatives Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN657824
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This SFPQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 676-705 AA from the C-terminal region of human SFPQ.
Clone RB34049
Isotype Ig
Specificity This SFPQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 675-705 amino acids from the C-terminal region of human SFPQ.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SFPQ
Background DNA-and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing, in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1, in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends, in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
Synonyms: Splicing factor, proline- and glutamine-rich,SFPQ,PSF
Molecular Weight 76149 DA
Gene ID 6421
UniProt NP_005057.1, P23246
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes Recommend dilutions: WB : 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment SFPQ Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Background publications Heyd, Lynch: "Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing." in: Molecular cell, Vol. 40, Issue 1, pp. 126-37, 2010 (PubMed).

Alternatives for antigen "Splicing Factor Proline/glutamine-Ric (SFPQ)", type "Antibodies"
Hosts (14), (12), (3)
Reactivities (26), (8), (6), (3), (2), (1), (1), (1)
Applications (26), (11), (8), (8), (6), (5), (2), (1), (1), (1), (1)
Epitopes (3), (3), (2), (2), (2), (1), (1), (1), (1)
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