CD44 (CD44) antibody

Details for Product No. ABIN659120
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Antigen
Synonyms AU023126, AW121933, AW146109, HERMES, Ly-24, Pgp-1, CD44A, METAA, RHAMM, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1
Reactivity
Human
(624), (322), (142), (80), (67), (58), (34), (16), (12), (12), (9), (8), (7), (3), (1)
Host
Mouse
(507), (199), (174), (9), (2), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(140), (82), (47), (27), (20), (9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(549), (268), (204), (149), (147), (124), (119), (74), (47), (40), (13), (6), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN659120
291.50 $
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Immunogen This CD44 monoclonal antibody is generated from mouse immunized with CD44 recombinant protein.
Clone Hermes-3
Isotype IgG2a, kappa
Specificity This CD44 monoclonal antibody is generated from mouse immunized with CD44 recombinant protein.
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Alternative Name CD44
Background The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Synonyms: LHR, MDU2, MDU3, MIC4,CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD_antigen=CD44, Flags: Precursor
Pathways: Hematopoietic cell lineage, ECM-receptor interaction, Shigellosis
Molecular Weight 81,538 Da
Gene ID 960
UniProt P16070
Research Area Cell Structure
Application Notes WB = 1:100-500, IHC = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-CD44 (CD44) antibody CD44 antibody confocal immunofluorescent analysis with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).
anti-CD44 (CD44) antibody (2) CD44 antibody western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD44 antibody detected the CD44 protein (arrow).
anti-CD44 (CD44) antibody (3) CD44 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD44 antibody for immunohistochemistry. Clinical relevance has not been evaluated.
anti-CD44 (CD44) antibody (4) CD44 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Background publications Wolny, Banerji, Gounou et al.: "Analysis of CD44-hyaluronan interactions in an artificial membrane system: insights into the distinct binding properties of high and low molecular weight hyaluronan." in: The Journal of biological chemistry, Vol. 285, Issue 39, pp. 30170-80, 2010 (PubMed).

Somasunderam, Thiviyanathan, Tanaka et al.: "Combinatorial selection of DNA thioaptamers targeted to the HA binding domain of human CD44." in: Biochemistry, Vol. 49, Issue 42, pp. 9106-12, 2010 (PubMed).

da Cunha, Oliveira, Wen et al.: "De novo expression of CD44 variants in sporadic and hereditary gastric cancer." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 90, Issue 11, pp. 1604-14, 2010 (PubMed).

Validation Images
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