CD44 antibody (CD44)

Details for Product anti-CD44 Antibody No. ABIN94121
Antigen
Reactivity
Human, Pig (Porcine), Dog (Canine)
792
341
169
47
42
42
35
31
29
26
16
14
11
10
10
6
5
4
2
1
1
1
1
1
1
1
1
1
Host
Mouse
638
222
198
12
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD44 antibody is un-conjugated
142
88
74
43
11
11
9
8
8
8
8
6
6
6
6
6
6
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
680
405
317
222
178
175
172
114
40
40
33
33
16
16
14
13
8
6
5
3
3
3
3
1
1
1
1
Options
Immunogen COS-7 cells (African Green Monkey).
Clone MEM-263
Isotype IgG1
Specificity The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative on platelets and hepatocytes.
HLDA III, WS Code T 155
Cross-Reactivity (Details) Human, Porcine, Canine (Dog)
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD44 (CD44 Antibody Abstract)
Background CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
Pathways Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD44 (CD44) antibody (ABIN94121) Flow cytometry analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (...
Image no. 2 for anti-CD44 (CD44) antibody (ABIN94121) Western Blotting peripheral blood lymphocytes of various species using anti-CD44 (MEM...
Product cited in: Klein, Weisshardt, Kleff, Jastrow, Jakob, Ergün: "Vascular wall-resident CD44+ multipotent stem cells give rise to pericytes and smooth muscle cells and contribute to new vessel maturation." in: PLoS ONE, Vol. 6, Issue 5, pp. e20540, 2011 (PubMed).