tRNA-YW Synthesizing Protein 1 Homolog (S. Cerevisiae) (TYW1) (C-Term) antibody

Details for Product No. ABIN783488
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Antigen
Synonyms RSAFD1, TYW1A, YPL207W, RGD1310420, Rsafd1, AW125391, im:6898509, wu:fb74d01, zgc:153368
Epitope
C-Term
(7), (3), (1)
Reactivity
Human, Rat (Rattus)
(12), (6)
Host
Rabbit
(9), (3)
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
(12), (7), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN783488
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Immunogen 19 amino acid peptide near the carboxy terminus of human TYW1
Specificity This antibody detects TYW1 / RSAFD1 at C-term. TYW1 antibody is predicted to not cross-react with other TYW protein family members. At least three isoforms of TYW1 are known to exist, this antibody will only detect the two larger isoforms.
Cross-Reactivity (Details) Species reactivity (tested):Human, rat
Purification Affinity chromatography purified via peptide column
Alternative Name TYW1 / RSAFD1
Background TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions.Synonyms: Radical S-adenosyl methionine and flavodoxin domain-containing protein 1, tRNA wybutosine-synthesizing protein 1 homolog, tRNA-yW-synthesizing protein
Gene ID 55253
NCBI Accession NP_060734
UniProt Q9NV66
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.
Background publications Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7. ..." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).

Noma, Kirino, Ikeuchi et al.: "Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA." in: The EMBO journal, Vol. 25, Issue 10, pp. 2142-54, 2006 (PubMed).

Noma, Suzuki: "Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis." in: Nucleic acids symposium series (2004), Issue 50, pp. 65-6, 2006 (PubMed).

Goto-Ito, Ishii, Ito et al.: "Structure of an archaeal TYW1, the enzyme catalyzing the second step of wye-base biosynthesis." in: Acta crystallographica. Section D, Biological crystallography, Vol. 63, Issue Pt 10, pp. 1059-68, 2007 (PubMed).

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