You are viewing an incomplete version of our website. Please click to reload the website as full version.

tRNA-YW Synthesizing Protein 1 Homolog (S. Cerevisiae) (TYW1) (C-Term) antibody

Details for Product No. ABIN783488, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms RSAFD1, TYW1A, YPL207W, RGD1310420, Rsafd1, AW125391, im:6898509, wu:fb74d01, zgc:153368
(5), (3), (3), (1)
Human, Rat (Rattus)
(11), (4)
(7), (4)
Enzyme Immunoassay (EIA), Western Blotting (WB)
(11), (7), (1), (1), (1)
Pubmed 4 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen 19 amino acid peptide near the carboxy terminus of human TYW1
Specificity This antibody detects TYW1 / RSAFD1 at C-term. TYW1 antibody is predicted to not cross-react with other TYW protein family members. At least three isoforms of TYW1 are known to exist, this antibody will only detect the two larger isoforms.
Cross-Reactivity (Details) Species reactivity (tested):Human, rat
Purification Affinity chromatography purified via peptide column
Alternative Name TYW1 / RSAFD1 (TYW1 Antibody Abstract)
Background TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions.Synonyms: Radical S-adenosyl methionine and flavodoxin domain-containing protein 1, tRNA wybutosine-synthesizing protein 1 homolog, tRNA-yW-synthesizing protein
Gene ID 55253
NCBI Accession NP_060734
UniProt Q9NV66
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-tRNA-YW Synthesizing Protein 1 Homolog (S. Cerevisiae) (TYW1) (C-Term) antibody (ABIN783488) Western blot analysis of TYW1 in human lung tissue lysate with TYW1 antibody at 1 μg/ml.
Background publications Goto-Ito, Ishii, Ito et al.: "Structure of an archaeal TYW1, the enzyme catalyzing the second step of wye-base biosynthesis." in: Acta crystallographica. Section D, Biological crystallography, Vol. 63, Issue Pt 10, pp. 1059-68, 2007 (PubMed).

Noma, Suzuki: "Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis." in: Nucleic acids symposium series (2004), Issue 50, pp. 65-6, 2006 (PubMed).

Noma, Kirino, Ikeuchi et al.: "Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA." in: The EMBO journal, Vol. 25, Issue 10, pp. 2142-54, 2006 (PubMed).

Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7. ..." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).

Catalog No. ABIN783488
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)