TYW1 antibody (C-Term)

Catalog No. ABIN6991540

Product Details anti-TYW1 Antibody

Target: TYW1 (tRNA-YW Synthesizing Protein 1 Homolog (TYW1))

Binding Specificity: AA 520-570, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TYW1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: TYW1 antibody is predicted to not cross-react with other TYW protein family members. At least three isoforms of TYW1 are known to exist, this antibody will only detect the two larger isoforms.

Purification: TYW1 Antibody is affinity chromatography purified via peptide column.

Immunogen: TYW1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TYW1. The immunogen is located within amino acids 520 - 570 of TYW1.

Isotype: IgG

Application Details

Application Notes: TYW1 antibody can be used for detection of TYW1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TYW1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: TYW1 antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TYW1

Target: TYW1 (tRNA-YW Synthesizing Protein 1 Homolog (TYW1))

Alternative Name: TYW1 (TYW1 Products)

Synonyms: RSAFD1 antibody, TYW1A antibody, YPL207W antibody, RGD1310420 antibody, Rsafd1 antibody, AW125391 antibody, im:6898509 antibody, wu:fb74d01 antibody, zgc:153368 antibody, tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) antibody, S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase antibody, tRNA-yW synthesizing protein 1 homolog S homeolog antibody, tRNA-yW synthesizing protein 1 homolog antibody, TYW1 antibody, LOC100354908 antibody, tyw1.S antibody, Tyw1 antibody, tyw1 antibody

Background: TYW1 Antibody: TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions

Gene ID: 55253

NCBI Accession: NP_060734

UniProt: Q9NV66